Nouioui Imen, Ghodhbane-Gtari Faten, Jando Marlen, Klenk Hans-Peter, Gtari Maher
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Institut Supérieur de Biotechnologie de Sidi Thabet, Université La Manouba, Manouba, Tunisia.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005656.
A nitrogen-fixing actinobacterium strain (Cc1.17) isolated from a root nodule of was subjected to polyphasic taxonomic studies. The strain was characterized by the presence of -diaminopimelic acid in its peptidoglycan, galactose, glucose, mannose, rhamnose, ribose and xylose as cell-wall sugars, phosphatidylinositol, diphosphatidylglycerol, glycophospholipids, phosphatidylglycerol, glycophospholipid and uncharacterized lipids as its polar lipids, and C, isoC, C ω9 and C 9 as major fatty acids (>10 %). Strain Cc1.17 showed 16S rRNA gene sequence similarities of 97.4-99.8 % to validly named species. Phylogenetic trees based on 16S rRNA gene and genome sequences placed strain Cc1.17 in a new lineage within the genus . Digital DNA-DNA hybridization and average nucleotide identity values between strain Cc1.17 and its closest phylogenomic neighbours were well below the thresholds recommended for prokaryotic species delineation. Therefore, strain Cc1.17 (=DSM 43829=CECT 9313) merits recognition as the type strain of a new species for which the name sp. nov. is proposed.
从[植物名称]根瘤中分离出的一株固氮放线菌菌株（Cc1.17）进行了多相分类研究。该菌株的特征在于其肽聚糖中存在 - 二氨基庚二酸，细胞壁糖中有半乳糖、葡萄糖、甘露糖、鼠李糖、核糖和木糖，其极性脂有磷脂酰肌醇、双磷脂酰甘油、糖脂、磷脂酰甘油、糖脂和未鉴定的脂类，主要脂肪酸（>10%）为C、异C、C ω9和C 9。菌株Cc1.17的16S rRNA基因序列与有效命名的[属名]物种的相似性为97.4 - 99.8%。基于16S rRNA基因和基因组序列构建的系统发育树将菌株Cc1.17置于[属名]属内的一个新谱系中。菌株Cc1.17与其最接近的系统发育邻居之间的数字DNA - DNA杂交和平均核苷酸同一性值远低于原核生物物种划分推荐的阈值。因此，菌株Cc1.17（=DSM 43829 = CECT 9313）应被认定为一个新物种的模式菌株，为此提出新物种名称[新物种名] sp. nov.。
