Kim Inhyup, Seo Taegun
Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005671.
A Gram-stain-positive, facultatively aerobic, rod-shaped and non-motile bacterial strain designated RMG13 was isolated from the soil near Gaetgol Eco Park and collected in Siheung-si, Republic of Korea. It was taxonomically characterized through polyphasic analysis. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel isolate was most closely related to the type strains of species of the genus . RMG13 shared the highest similarities with ALL (99.2 %) and YJ56 (99.0 %). DNA-DNA hybridization values of RMG13 with ALL and YJ56 were 28.1 and 41.8 %, respectively. The average nucleotide identities of RMG13 with ALL and YJ56 were 84.2 and 70.3 %, respectively, whilst the average amino acid identities of RMG13 with ALL and YJ56 were 90.5 and 74.6 %, respectively. Two-dimensional thin-layer chromatography showed that the major polar lipids of RMG13 were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, and its major cellular fatty acids were anteiso-C, iso-C and anteiso-C. MK-9 (H2) was the sole menaquinone. Cells grew at 4-37 °C (optimum 30 °C) and pH 5.0-12.0 (optimum pH 8.0) in Reasoner's 2A (MB cell). The cells tolerated 0-5% NaCl (w/v) but not 6 % NaCl. The DNA G+C content of RMG13 was 65.0 %. The results of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses indicated that RMG13 represents a novel member of the genus , and the proposed name is sp. nov. The type strain of is RMG13 (= KACC 22359 = TBRC 15115).
从韩国始兴市加德戈尔生态公园附近土壤中分离出一株革兰氏阳性、兼性需氧、杆状且无运动性的细菌菌株，命名为RMG13。通过多相分析对其进行了分类学特征鉴定。基于16S rRNA基因的系统发育分析表明，该新分离菌株与[属名]属物种的模式菌株关系最为密切。RMG13与ALL（99.2%）和YJ56（99.0%）的相似性最高。RMG13与ALL和YJ56的DNA-DNA杂交值分别为28.1%和41.8%。RMG13与ALL和YJ56的平均核苷酸同一性分别为84.2%和70.3%，而RMG13与ALL和YJ56的平均氨基酸同一性分别为90.5%和74.6%。二维薄层色谱显示，RMG13的主要极性脂质为双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇，其主要细胞脂肪酸为anteiso-C、iso-C和anteiso-C。MK-9（H2）是唯一的甲基萘醌。细胞在Reasoner's 2A（MB细胞）培养基中于4-37°C（最适温度30°C）和pH 5.0-12.0（最适pH 8.0）下生长。细胞能耐受0-5%（w/v）的NaCl，但不能耐受6%的NaCl。RMG13的DNA G+C含量为65.0%。表型、基因型、化学分类学和系统发育分析结果表明，RMG13代表[属名]属的一个新成员，提议的名称为[属名] sp. nov.。[属名]的模式菌株为RMG13（=KACC 22359=TBRC 15115）。
