Wu Zhang-Ping, Zheng Xi-Wen, Sun Ya-Ping, Wang Bei-Bei, Hou Jing, Cui Heng-Lin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005637.
A filamentous cell-shaped halophilic archaeon (strain AD-1) was isolated from Aiding Salt Lake, PR China. Its colonies on HCM7 agar plates were pinkish white, 1-4 mm (diameter), elevated and round. The optimum conditions for growth were observed at 42 °C, 4.3 M NaCl, 0.01 M MgCl and pH 7. Strain AD-1 could hydrolyse Tween 60, Tween 80, starch and gelatin. Phylogenetic analysis based on 16S rRNA gene, rpoB' and the concatenated 484 single-copy orthologous proteins revealed that strain AD-1 formed a clade with SPP-AMP-1. The average nucleotide identity and DNA-DNA hybridization values between strain AD-1 and SPP-AMP-1 were both below the species delineation thresholds (95~96 and 70 %, respectively). The major phospholipids of strain AD-1 were phosphatidic acid, phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, while the major glycolipids were sulphated galactosyl mannosyl glucosyl diether, galactosyl mannosyl glucosyl diether and glucosyl mannosyl glucosyl diether. The phenotypic, phylogenetic and genome-based analyses suggested that strain AD-1 (=CGMCC 1.13724=JCM 32960) represents a novel species, for which the name sp. nov. is proposed.
从中国新疆艾丁湖分离出一株丝状细胞形态的嗜盐古菌（菌株AD-1）。其在HCM7琼脂平板上的菌落为粉白色，直径1-4毫米，凸起且呈圆形。观察到其生长的最佳条件为42℃、4.3M氯化钠、0.01M氯化镁和pH7。菌株AD-1能够水解吐温60、吐温80、淀粉和明胶。基于16S rRNA基因、rpoB'和串联的484个单拷贝直系同源蛋白的系统发育分析表明，菌株AD-1与SPP-AMP-1形成一个进化枝。菌株AD-1与SPP-AMP-1之间的平均核苷酸同一性和DNA-DNA杂交值均低于物种划分阈值（分别为95~96%和70%）。菌株AD-1的主要磷脂为磷脂酸、磷脂酰甘油和磷脂酰甘油磷酸甲酯，而主要糖脂为硫酸化半乳糖基甘露糖基葡萄糖基二醚、半乳糖基甘露糖基葡萄糖基二醚和葡萄糖基甘露糖基葡萄糖基二醚。表型、系统发育和基于基因组的分析表明，菌株AD-1（=CGMCC 1.13724=JCM 32960）代表一个新物种，为此提出新种名sp. nov. 。
