NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, International Center for Technology and Innovation of Animal Model, Comparative Medicine Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, 100021, China.
Changping National Laboratory (CPNL), Beijing, 102299, China.
Arch Microbiol. 2023 Apr 5;205(5):169. doi: 10.1007/s00203-023-03513-z.
A Gram-negative strain, anaerobic, non-motile, non-spore-forming, rod-shaped bacterial strain named as NGMCC 1.200684 was isolated from the fresh feces of rhinoceros in Beijing Zoo. Based on 16S rRNA gene sequences, phylogenetic analysis indicated that strain NGMCC 1.200684 belonged to the genus Bacteroides and was most strongly related to the type strain of Bacteroides uniformis ATCC 8492 (96.88%). The G + C content of the genomic DNA was determined to be 46.62%. Between strains NGMCC 1.200684 and B. uniformis ATCC 8492, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) were 93.89 and 67.60%, respectively. Strain NGMCC 1.200684 can produce acid from fermentation of several substrates, including glucose, mannitol, lactose, saccharose, maltose, salicin, xylose, cellobiose, mannose, raffinose, sorbitol, trehalose, D-galactose, and maltotriose. The major cellular fatty acids (> 10%) were identified as anteiso-C, iso-C, iso-C, and iso-C 3-OH. The polar lipid profiles of strain NGMCC 1.200684 were determined to contain diphosphatidyl glycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, and two unknown amino-phospholipids. Based on phenotypic, phylogenetic, and chemotaxonomic characteristics, a novel species of the genus Bacteroides, Bacteroides rhinocerotis sp. nov. is proposed. The type strain is NGMCC 1.200684 (= CGMCC 1.18013 = JCM 35702).
从北京动物园的新鲜犀牛粪便中分离到一株革兰氏阴性、厌氧、非运动、非孢子形成的杆状细菌菌株，命名为 NGMCC 1.200684。基于 16S rRNA 基因序列，系统发育分析表明，菌株 NGMCC 1.200684 属于拟杆菌属，与模式菌株均匀拟杆菌 ATCC 8492（96.88%）最为密切相关。基因组 DNA 的 G+C 含量为 46.62%。在 NGMCC 1.200684 菌株和 B. uniformis ATCC 8492 菌株之间，平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）分别为 93.89%和 67.60%。菌株 NGMCC 1.200684 可以从葡萄糖、甘露醇、乳糖、蔗糖、麦芽糖、水杨苷、木糖、纤维二糖、甘露糖、棉子糖、山梨醇、海藻糖、D-半乳糖和麦芽三糖等几种底物的发酵中产生酸。主要的细胞脂肪酸（>10%）鉴定为 anteiso-C、iso-C、iso-C 和 iso-C 3-OH。菌株 NGMCC 1.200684 的极性脂谱确定含有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未知磷脂和两种未知氨基磷脂。基于表型、系统发育和化学分类学特征，提出了拟杆菌属的一个新种，即拟杆菌犀牛种。模式菌株为 NGMCC 1.200684（=CGMCC 1.18013=JCM 35702）。
