Wang Maidi, Du Jingtao, Li Mengya, Pierini Filippo, Li Xiaoran, Yu Jianyong, Ding Bin
Innovation Center for Textile Science and Technology, College of Textiles, Donghua University, Shanghai 201620, China.
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw 02-106, Poland.
Biomater Sci. 2023 Mar 28;11(7):2383-2394. doi: 10.1039/d2bm01891h.
forming injectable hydrogels hold great potential for the treatment of irregular wounds. However, their practical applications were hindered by long gelation time, poor mechanical performance, and a lack of a natural extracellular matrix structure. Herein, amino-modified electrospun poly(lactic--glycolic acid) (APLGA) short fibers with uniform distribution were introduced into gelatin methacrylate/oxidized dextran (GM/ODex) hydrogels. In comparison with the fiber aggregation structure in the PLGA fiber-incorporated hydrogels, the hydrogels with APLGA fibers possessed a uniform porous structure. The highly dispersed APLGA short fibers accelerated the sol-gel phase transition of the hydrogel due to the formation of dynamic Schiff-base bonds between the fibers and hydrogels. Furthermore, in combination with UV-assisted crosslinking, a rapid gelation time of 90 s was achieved for the double-crosslinked hydrogels. The addition of APLGA short fibers as fillers and the formation of the double-crosslinking network enhanced the mechanical performance of the hydrogels. Furthermore, the fiber-hydrogel composites exhibited favorable injectability, excellent biocompatibility, and improved cell infiltration. assessment indicated that the GM/ODex-APLGA hydrogels successfully filled the full-thickness defects and improved wound healing. This work demonstrates a promising solution for the treatment of irregular wounds.
可注射水凝胶在不规则伤口治疗方面具有巨大潜力。然而，其实际应用受到凝胶化时间长、力学性能差以及缺乏天然细胞外基质结构的阻碍。在此，将具有均匀分布的氨基改性电纺聚乳酸-乙醇酸共聚物（APLGA）短纤维引入甲基丙烯酸明胶/氧化葡聚糖（GM/ODex）水凝胶中。与掺入PLGA纤维的水凝胶中的纤维聚集结构相比，含有APLGA纤维的水凝胶具有均匀的多孔结构。高度分散的APLGA短纤维由于在纤维与水凝胶之间形成动态席夫碱键而加速了水凝胶的溶胶-凝胶相变。此外，结合紫外线辅助交联，双交联水凝胶的凝胶化时间缩短至90秒。添加APLGA短纤维作为填料以及形成双交联网络增强了水凝胶的力学性能。此外，纤维-水凝胶复合材料表现出良好的可注射性、优异的生物相容性和改善的细胞浸润性。评估表明，GM/ODex-APLGA水凝胶成功填充了全层缺损并促进了伤口愈合。这项工作为不规则伤口的治疗展示了一个有前景的解决方案。
