一种用于地塞米松治疗期间过氧亚硝酸盐成像的长波长线粒体靶向荧光探针。
A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment.
作者信息
Tang Jun, Li Ziyi, Qiang Chuchu, Han Yan, Yang Lifang, Zhu Li, Dang Tan, Chen Gairong, Ye Yong
机构信息
School of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, Henan 453003, China.
School of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, Henan 453003, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 5;292:122429. doi: 10.1016/j.saa.2023.122429. Epub 2023 Feb 3.
Peroxynitrite (ONOO), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence "turn on" probe TL. The probe TL could react with ONOO by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO, thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO. This indicated that TL has excellent potential to study the fluctuation of ONOO in the physiological and pathological system.
过氧亚硝酸盐（ONOO）作为一种强氧化性活性氮物质（RNS），由细胞内源性产生。其可视化研究对于理解相关疾病过程至关重要。在此，我们报道了一种长波长线粒体靶向荧光“开启”探针TL。探针TL以4-（溴甲基）苯硼酸为反应位点与ONOO发生反应，在检测ONOO方面表现出优异的特性，从而改善了响应时间（约50秒）、灵敏度（检测限，10.1 nM）和发射波长（667 nm）。此外，TL在生物系统中对外源性和内源性ONOO表现出良好的线粒体靶向性和生物可视化能力。最后，TL用于监测高浓度地塞米松诱导的ONOO上调。这表明TL在研究生理和病理系统中ONOO的波动方面具有优异的潜力。
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