Rodríguez T, Tormo B, Rengifo E, Grá B, González N, Mandado S
National Institute of Oncology and Radiobiology, Havana, Cuba.
Arch Geschwulstforsch. 1987;57(4):287-96.
Fine structure and function are closely related in the cells of the different tissues. For this reason a comparative study combining histochemical techniques and scanning electron microscopy can result a potentially useful tool in the search for cell markers of preneoplastic stages of the colon mucosa. With this purpose, samples of normal colon mucosa, adenomatous polyps and adenocarcinomas obtained by colonoscopy were studied by histochemical techniques for the detection of epithelial mucosubstances and by scanning electron microscopy. Differences were shown in the architectural arrangement of the colon epithelium between the normal mucosa, the adenocarcinoma and the polyps studied, while between tubular an tubulovillous adenomas no marked differential ultrastructural features were found. Acid mucosubstances distribution pattern showed that sialomucin predominated in the colon mucosa harboring a carcinoma and in the tubulo-villous polyps as well as in the mucosa remote from adenocarcinoma and from the two cases of familial polyposis coli histologically considered as tubulo-villous adenomas. On the other hand tubular adenomas and normal mucosa showed a predominance of sulfomucin.
在不同组织的细胞中，精细结构与功能密切相关。因此，将组织化学技术与扫描电子显微镜相结合的比较研究，可能成为寻找结肠黏膜肿瘤前期细胞标志物的有用工具。为此，通过组织化学技术检测上皮黏液物质，并利用扫描电子显微镜，对通过结肠镜检查获得的正常结肠黏膜、腺瘤性息肉和腺癌样本进行了研究。在所研究的正常黏膜、腺癌和息肉之间，结肠上皮的结构排列存在差异，而在管状腺瘤和管状绒毛状腺瘤之间未发现明显的超微结构差异特征。酸性黏液物质的分布模式表明，涎黏蛋白在患有癌的结肠黏膜、管状绒毛状息肉以及远离腺癌的黏膜中占主导地位，在两例组织学上被视为管状绒毛状腺瘤的家族性结肠息肉病病例中也是如此。另一方面，管状腺瘤和正常黏膜中硫黏蛋白占主导地位。
