Mughal S, Filipe M I
J Natl Cancer Inst. 1978 Apr;60(4):753-68. doi: 10.1093/jnci/60.4.753.
Ultrastructural changes that occurred in the colon epithelia of patients with familial polyposis coli were investigated. Criteria of gradation of the crescendo changes from the mucosa in the controls, through the "normal" mucosa between polyps and adenomas in various stages of dedifferentiation, to invasive carcinoma were established. Our criteria were based on the following requirements: a) vesiculation and increasing numbers of small electron-dense bodies (secretory granules) and lysosomes in the mature and immature absorptive cells, b) presence of immature and undifferiated cells, c) variation in the globlet cells and appearance of atypical secretory cells, and d) nuclear changes. The results illustrated the adenoma-carcinoma sequence and added strong evidence to support its occurrence. Furthermore, this ultrastructural study revealed cellular changes that preceded adenomatous growth and may be of value as markers of early stages of cancer. However, this study also revealed a close link between the function and morphology of the mucosal epithelium.
对家族性结肠息肉病患者结肠上皮的超微结构变化进行了研究。确立了从对照组黏膜的渐进性变化，经过不同去分化阶段息肉与腺瘤之间的“正常”黏膜，直至浸润性癌的分级标准。我们的标准基于以下要求：a）成熟和未成熟吸收细胞中出现小泡形成以及小电子致密体（分泌颗粒）和溶酶体数量增加；b）存在未成熟和未分化细胞；c）杯状细胞的变化以及非典型分泌细胞的出现；d）细胞核变化。结果阐明了腺瘤 - 癌序列，并为其发生提供了有力证据。此外，这项超微结构研究揭示了腺瘤性生长之前的细胞变化，可能作为癌症早期阶段的标志物具有价值。然而，这项研究还揭示了黏膜上皮功能与形态之间的密切联系。
