National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Faculty of Medical Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran.
J Biomed Mater Res A. 2023 Aug;111(8):1216-1227. doi: 10.1002/jbm.a.37509. Epub 2023 Feb 8.
The human amniotic membrane dressing has been shown to accelerate the wound healing process in the clinic. In this study, heparin was conjugated to a human Acellular Amniotic Membrane (hAAM) to provide affinity binding sites for immobilizing growth factors. To study the acceleration of the wound healing process, we bound epidermal growth factor and fibroblast growth factor 1 to heparinized hAAMs (GF-Hep-hAAMs). The heparinized hAAMs (Hep-hAAMs) were characterized by toluidine blue staining and infrared spectroscopy. The quality control of hAAM was performed by hematoxylin staining, swelling capacity test and biomechanical evaluation. The cytotoxicity, adhesion, and migration in vitro assays of GF-Hep-hAAMs on L-929 fibroblast cells were also studied by MTT assay, scanning electron microscopy, and scratch assay, respectively. Finally, in vivo skin wound healing study was performed to investigate the wound closure rate, re-epithelization, collagen deposition, and formation of new blood vessels. The results showed that GF-Hep-hAAMs enhance the rate of wound closure and epidermal regeneration in BALB/c mice. In conclusion, GF-Hep-hAAMs could accelerate the wound healing process, significantly in the first week.
人羊膜敷料已被证明可在临床上加速伤口愈合过程。在这项研究中，肝素被连接到人去细胞羊膜（hAAM）上，以提供用于固定生长因子的亲和结合位点。为了研究伤口愈合过程的加速，我们将表皮生长因子和纤维母细胞生长因子 1 结合到肝素化 hAAMs（GF-Hep-hAAMs）上。肝素化 hAAMs（Hep-hAAMs）通过甲苯胺蓝染色和红外光谱进行表征。通过苏木精染色、溶胀能力测试和生物力学评估对 hAAM 进行质量控制。通过 MTT 测定、扫描电子显微镜和划痕测定分别研究了 GF-Hep-hAAMs 在 L-929 成纤维细胞上的细胞毒性、黏附和迁移的体外测定。最后，进行体内皮肤伤口愈合研究，以研究伤口闭合率、再上皮化、胶原蛋白沉积和新血管形成。结果表明，GF-Hep-hAAMs 可提高 BALB/c 小鼠的伤口闭合率和表皮再生率。总之，GF-Hep-hAAMs 可显著加速伤口愈合过程，尤其是在第一周。
