Cilingir Sumeyye, Acikel-Elmas Merve, Arbak Serap, Kolgazi Meltem
Institute of Health Sciences, Acibadem Mehmet Ali Aydınlar University, Icerenkoy Mah., Kayisdagi Cad. No: 32, Atasehir, 34752, Istanbul, Turkey.
Department of Histology and Embryology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Icerenkoy Mah., Kayisdagi Cad. No: 32, Atasehir, 34752, Istanbul, Turkey.
Inflammopharmacology. 2023 Apr;31(2):997-1008. doi: 10.1007/s10787-023-01150-y. Epub 2023 Feb 8.
Acute pancreatitis is a systemic inflammatory disorder characterized by the hyperactivation of digestion enzymes and the release of proinflammatory cytokines. Ferulic acid (FA) is a hydroxycinnamic acid derivative that has recently been shown to have antioxidant and anti-inflammatory properties.
The anti-inflammatory effects of FA were investigated in the pancreaticobiliary duct ligation (PBDL)-induced pancreatitis model.
Wistar albino rats (250-300 g; female = male) were divided into sham operation and PBDL groups. Some PBDL-performed animals were given intragastric saline or 250 mg/kg FA or 500 mg/kg FA 30 min before the PBDL and for 3 consecutive days. Moreover, the control group received saline. Blood samples are collected at the 24th, 48th, and 72nd hours to measure serum tumor necrosis factor (TNF)-α, liver, and pancreatic enzymes. At the 72nd hour, rats were euthanized; pancreas, lung, and liver samples were collected, scored microscopically, and analyzed for myeloperoxidase activity, malondialdehyde, and glutathione levels. One-way ANOVA with Tukey-Kramer tests were used for statistical analysis.
FA treatment reduced myeloperoxidase activity and prevented the depletion of glutathione in all three tissues. With FA treatments, high malondialdehyde levels in the pancreas and liver were reduced, as were serum TNF- α, amylase, lipase, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels. Additionally, FA ameliorated microscopic damage in the pancreas and liver significantly.
According to the findings, FA protects endogenous antioxidant content, prevents neutrophil infiltration, and decreases lipid peroxidation in PBDL-induced pancreatitis. Furthermore, FA improves tissue damage induced by pancreatitis with its anti-inflammatory effects.
急性胰腺炎是一种全身性炎症性疾病，其特征在于消化酶的过度活化和促促促炎细胞因子的释放。阿魏酸（FA）是一种羟基肉桂酸衍生物，最近已被证明具有抗氧化和抗炎特性。
在胰胆管结扎（PBDL）诱导的胰腺炎模型中研究FA的抗炎作用。
将Wistar白化大鼠（250 - 300克；雌性 = 雄性）分为假手术组和PBDL组。一些接受PBDL手术的动物在PBDL前30分钟给予胃内生理盐水或250毫克/千克FA或500毫克/千克FA，并连续3天给药。此外，对照组接受生理盐水。在第24、48和72小时采集血样，以测量血清肿瘤坏死因子（TNF）-α、肝脏和胰腺酶。在第72小时，对大鼠实施安乐死；收集胰腺、肺和肝脏样本，进行显微镜评分，并分析髓过氧化物酶活性、丙二醛和谷胱甘肽水平。采用单向方差分析和Tukey-Kramer检验进行统计分析。
FA治疗降低了所有三种组织中的髓过氧化物酶活性，并防止了谷胱甘肽的消耗。通过FA治疗，胰腺和肝脏中高丙二醛水平降低，血清TNF-α、淀粉酶、脂肪酶、碱性磷酸酶、丙氨酸转氨酶、天冬氨酸转氨酶和总胆红素水平也降低。此外，FA显著改善了胰腺和肝脏的微观损伤。
根据研究结果，FA可保护内源性抗氧化剂含量，防止中性粒细胞浸润，并减少PBDL诱导的胰腺炎中的脂质过氧化。此外，FA通过其抗炎作用改善胰腺炎诱导的组织损伤。
