Nature Research Centre, Akademijos Str. 2, Vilnius, 08412, Lithuania.
Kathrinenhof Research Center, Boehringer Ingelheim Vetmedica GmbH, Walchenseestr. 8-12, 83101, Rohrdorf, Germany.
Vet Res Commun. 2023 Dec;47(4):2165-2171. doi: 10.1007/s11259-023-10079-0. Epub 2023 Feb 8.
In this study, for the first time, Sarcocystis species were identified molecularly in sika deer (Cervus nippon) that form free-ranging populations in several countries of Europe. Diaphragm muscle samples from 151 deer from 10 populations in Germany and Austria were examined for sarcocysts. By one-gram methylene-blue staining, sarcocysts were recorded in samples of 114 animals (75.5%) which originated from all populations. Sarcocysts were more often (p < 0.0001) recorded in yearling and adult deer than in calves. Infection intensity was generally low with ~ 70% of the sarcocyst positive deer harbouring ≤ 10 sarcocysts per 1-gram diaphragm muscle. Based on cox1 sequence comparison, 10 species of Sarcocystis, all previously reported parasitizing cervids, were identified: S. elongata, S. entzerothi, S. hjorti, S. iberica, S. japonica, S. linearis, S. morae, S. pilosa, S. silva and S. truncata. The prevailing S. hjorti was detected in sika deer of all 10 populations. The identification in sika deer of S. hjorti, S. iberica, S. elongata, S. linearis, S. morae and S. silva constitutes new host records. With the additional species records of this study, the highest number of Sarcocystis species, at least 16, was identified in this host.
在这项研究中，首次在欧洲多个国家自由放养的梅花鹿（ Cervus nippon ）中通过分子方法鉴定了 Sarcocystis 物种。对来自德国和奥地利 10 个种群的 151 只鹿的膈肌样本进行了 sarcocysts 检查。通过一克亚甲蓝染色，记录了来自所有种群的 114 只动物（75.5%）样本中的 sarcocysts。sarcocysts 在一岁龄和成年鹿中比在小牛中更常见（p<0.0001）。感染强度通常较低，约 70%的 sarcocyst 阳性鹿每 1 克膈肌肌肉中携带≤10 个 sarcocysts。基于 cox1 序列比较，鉴定出了 10 种 Sarcocystis ，均为先前报道的鹿寄生虫：S. elongata 、 S. entzerothi 、 S. hjorti 、 S. iberica 、 S. japonica 、 S. linearis 、 S. morae 、 S. pilosa 、 S. silva 和 S. truncata 。在所有 10 个种群的梅花鹿中均检测到优势 S. hjorti 。在梅花鹿中鉴定出 S. hjorti 、 S. iberica 、 S. elongata 、 S. linearis 、 S. morae 和 S. silva 构成了新的宿主记录。通过本研究的附加种记录，在这种宿主中至少鉴定出了 16 种 Sarcocystis ，这是数量最多的。
