Toledano Manuel, Osorio Estrella, Osorio María T, Aguilera Fátima S, Toledano Raquel, Romero Enrique Fernández-, Osorio Raquel
Faculty of Dentistry, Dental Materials Section, Colegio Máximo de Cartuja s/n, University of Granada, Granada 18071, Spain.
Faculty of Dentistry, Dental Materials Section, Colegio Máximo de Cartuja s/n, University of Granada, Granada 18071, Spain.
J Dent. 2023 Mar;130:104447. doi: 10.1016/j.jdent.2023.104447. Epub 2023 Feb 6.
Bioactive materials have been used for functionalization of adhesives to promote dentin remineralization. This study aims to evaluate bonding ability and both mechanical and chemical behavior of demineralized dentin infiltrated with polymeric nanoparticles doped with dexamethasone (Dex-NPs).
Dentin conditioned surfaces were infiltrated with NPs, Dex-NPs or Dex-Zn-NPs. Bonded interfaces were also created and stored for 24 h or 21d, and then submitted to microtensile bond strength testing. Dentin remineralization was analyzed by Nanohardness, Young's modulus and Raman analysis.
At 21d of storage, dentin treated with undoped-NPs attained the lowest nanohardness and Young's modulus. Dex-NPs and Zn-Dex-NPs increased dentin nanohardness and Young's modulus after 21d Raman analysis showed high remineralization, crystallinity, crosslinking and better structure of collagen when functionalized Dex-NPs were present at the dentin interface.
Infiltration of dentin with Dex-NPs promoted functional remineralization as proved by nanomechanical and morpho-chemical evaluation tests. Dexamethasone in dentin facilitated crystallographic maturity, crystallinity and improved maturity and secondary structure of dentin collagen.
Using dexamethasone-functionalized NPs before resin infiltration is a clear option to obtain dentin remineralization, as these NPs produce the reinforcement of the dentin structure, which will lead to the improvement of the longevity of resin restorations.
生物活性材料已被用于粘合剂的功能化，以促进牙本质再矿化。本研究旨在评估用掺杂地塞米松的聚合物纳米颗粒（Dex-NPs）浸润的脱矿牙本质的粘结能力以及机械和化学行为。
用纳米颗粒、Dex-NPs或Dex-Zn-NPs浸润牙本质处理过的表面。还创建粘结界面并储存24小时或21天，然后进行微拉伸粘结强度测试。通过纳米硬度、杨氏模量和拉曼分析来分析牙本质再矿化情况。
在储存21天时，用未掺杂纳米颗粒处理的牙本质的纳米硬度和杨氏模量最低。21天后，Dex-NPs和Zn-Dex-NPs提高了牙本质的纳米硬度和杨氏模量。拉曼分析表明，当牙本质界面存在功能化的Dex-NPs时，再矿化程度高、结晶度高、交联性好且胶原结构更好。
纳米力学和形态化学评估测试证明，用Dex-NPs浸润牙本质可促进功能性再矿化。牙本质中的地塞米松促进了晶体成熟度、结晶度，并改善了牙本质胶原的成熟度和二级结构。
在树脂浸润前使用地塞米松功能化的纳米颗粒是实现牙本质再矿化的明确选择，因为这些纳米颗粒可增强牙本质结构，从而提高树脂修复体的使用寿命。
