Toledano Manuel, Fernández-Romero Enrique, Aguilera Fátima S, Osorio Estrella, Rodríguez-Santana José A, Garrido Macarena, Solís Pedro A, García-Godoy Franklin, Osorio Raquel
University of Granada, Faculty of Dentistry. Colegio Máximo de Cartuja s/n, Granada 18071, Spain.
University of Granada, Faculty of Dentistry. Colegio Máximo de Cartuja s/n, Granada 18071, Spain; Medicina Clínica y Salud Pública PhD Programme, University of Granada, 18071 Granada, Spain.
J Dent. 2024 Sep;148:105027. doi: 10.1016/j.jdent.2024.105027. Epub 2024 Apr 26.
This study targets to assess the remineralization capability of conditioned dentin infiltrated with polymeric nanoparticles (NPs) doped with tideglusib (TDg) (TDg-NPs).
Dentin conditioned surfaces were infiltrated with NPs and TDg-NPs. Bonded interfaces were created, stored for 24 h and submitted to mechanical and thermal challenging. Resin-dentin interfaces were evaluated through nanohardness, Masson's trichrome staining microscopy, and Raman analysis.
Dentin surfaces treated with TDg-NPs and load cycled produced higher nanohardness than the rest of the groups at the hybrid layer. At the bottom of the hybrid layer, all samples treated with TDg-NPs showed higher nanohardness than the rest of the groups. Active remineralization underneath the hybrid layer was detected in all groups after TDg application and load cycling, inducting new dentinal tubuli formation. After thermocycling, remineralization at the hybrid layer was not evidenced in the absence of NPs. Raman analysis showed increase mineralization, enriched carbonate apatite formation, and improved crosslinking and scaffolding of the collagen.
Mechanical loading on the specimens obtained after TDg-NPs dentin infiltration inducts an increase of mineralization at the resin/dentin interface, indicating remineralization of peritubular and intertubular dentin with augmented crystallographic maturity in crystals. Enriched collagen quality was produced, generating an adequate matrix organization to promote apatite nucleation, after tideglusib infiltration.
At the present research, it has been proved the creation of reparative dentin, at the resin-dentin interface, after tideglusib dentin infiltration. Chemical stability, to favor integrity of the resin-dentin interface, is warranted in the presence of the TDg-NPs in the demineralized dentin collagen.
本研究旨在评估用掺杂替格瑞洛（TDg）的聚合物纳米颗粒（NPs）（TDg-NPs）浸润的预处理牙本质的再矿化能力。
用NPs和TDg-NPs浸润牙本质预处理表面。创建粘结界面，储存24小时，并进行机械和热挑战。通过纳米硬度、马松三色染色显微镜和拉曼分析评估树脂-牙本质界面。
用TDg-NPs处理并进行加载循环的牙本质表面在混合层产生了比其他组更高的纳米硬度。在混合层底部，所有用TDg-NPs处理的样品显示出比其他组更高的纳米硬度。在应用TDg和加载循环后，所有组在混合层下方均检测到活性再矿化，诱导新的牙本质小管形成。热循环后，在没有NPs的情况下，混合层未出现再矿化。拉曼分析显示矿化增加、富碳酸盐磷灰石形成以及胶原蛋白的交联和支架改善。
TDg-NPs牙本质浸润后获得的标本上的机械加载导致树脂/牙本质界面矿化增加，表明管周和管间牙本质再矿化，晶体的晶体学成熟度增加。替格瑞洛浸润后产生了富集的胶原蛋白质量，产生了适当的基质组织以促进磷灰石成核。
在本研究中，已证明替格瑞洛牙本质浸润后在树脂-牙本质界面形成了修复性牙本质。在脱矿牙本质胶原蛋白中存在TDg-NPs的情况下，保证化学稳定性以有利于树脂-牙本质界面的完整性是必要的。
