Xie Chenyang, Li Jie, Fang Yihe, Ma Renyi, Dang Zhixiong, Yang Fang
School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, China.
Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan, China.
J Sci Food Agric. 2023 May;103(7):3659-3673. doi: 10.1002/jsfa.12480. Epub 2023 Feb 21.
Chestnut (Castanea mollissima) shell is rich in flavonoids and our previous studies showed that proanthocyanins and anthocyanins were the two markedly varied flavonoids in chestnut shell extracts (CSE) during digestion. Here, the biotransformation of proanthocyanins and anthocyanins in a simulated gastrointestinal model, and the interactions between non-absorption CSE (NACSE) and gut microbiota in vitro, were investigated by ultra-high-performance liquid chromatography combined with triple-quadrupole mass spectrometry and 16S rRNA sequencing.
Chestnut shell was richer in proanthocyanins and anthocyanins, while the loss of proanthocyanins was greater after digestion. Additionally, the content of anthocyanin decreased after gastric digestion but increased after intestinal digestion and remained stable after fermentation. After fermentation, delphinidin-3-O-sambubioside and pelargonidin-3-O-galactoside were newly formed. Furthermore, microbiome profiling indicated that NACSE promoted the proliferation of beneficial bacteria, while inhibiting pathogenic bacteria.
All these data suggest that CSE may be a promising candidate to protect gut health. © 2023 Society of Chemical Industry.
板栗（Castanea mollissima）壳富含黄酮类化合物，我们之前的研究表明，原花青素和花青素是板栗壳提取物（CSE）在消化过程中两种显著变化的黄酮类化合物。在此，通过超高效液相色谱结合三重四极杆质谱和16S rRNA测序，研究了原花青素和花青素在模拟胃肠道模型中的生物转化，以及非吸收性CSE（NACSE）与体外肠道微生物群之间的相互作用。
板栗壳中富含原花青素和花青素，但消化后原花青素的损失更大。此外，花青素含量在胃消化后降低，但在肠道消化后增加，发酵后保持稳定。发酵后，新形成了飞燕草素-3-O-山梨糖苷和天竺葵素-3-O-半乳糖苷。此外，微生物群落分析表明，NACSE促进有益菌的增殖，同时抑制病原菌。
所有这些数据表明，CSE可能是保护肠道健康的一个有前景的候选物。©2023化学工业协会。
