Li Na, Wang Dan, Wen Xingjian, Chu Rui, Fan Jiuyu, Chen Yilong, Luo Yanfeng
College of Bioengineering, Chongqing University, Chongqing, China.
Chongqing Academy of Chinese Materia Medica, Chongqing, China.
J Sci Food Agric. 2023 May;103(7):3390-3401. doi: 10.1002/jsfa.12491. Epub 2023 Feb 24.
Cyclophosphamide (CTX) is a widely used chemotherapeutic agent for the treatment of malignant tumors and autoimmune diseases. However, it can cause immunosuppression and damage the intestinal mucosa. The development of new agents to counteract these side effects is becoming increasingly important. Previous studies have shown that the polysaccharides from Gastrodia elata (GEPs) have strong immune-enhancing effects; however, their functions regarding the intestines and the underlying mechanism are still unclear. In this study, the effects of GEPs on immunomodulatory activity, intestinal barrier function, and gut microbiota regulation were investigated in a mouse model of CTX-induced immunosuppression.
Gastrodia elata polysaccharides attenuated the CTX-induced decrease in organ indices of the thymus and spleen, and promoted the secretion of immune-related cytokines and immunoglobulins in the serum. They also improved the intestinal pathology and restored the intestinal barrier function by elevating the expression of intestinal tight junction proteins, occludin and ZO-1. Moreover, GEPs restored the composition and abundance of the gut microbiota and increased the short-chain fatty acid (SCFA) content in the colon. The abundance of SCFA-producing bacteria (Muribaculaceae, Prevotellaceae, and Bacteroidaceae) also increased.
Gastrodia elata polysaccharides can effectively alleviate immunosuppression and regulate the intestinal barrier integrity and the structure of gut microbiota in CTX-treated mice. They may be used as ingredients to develop functional foods for intestinal health. © 2023 Society of Chemical Industry.
环磷酰胺（CTX）是一种广泛用于治疗恶性肿瘤和自身免疫性疾病的化疗药物。然而，它会导致免疫抑制并损害肠黏膜。开发新的药物来对抗这些副作用变得越来越重要。先前的研究表明，天麻多糖（GEPs）具有强大的免疫增强作用；然而，它们在肠道方面的功能及其潜在机制仍不清楚。在本研究中，在CTX诱导的免疫抑制小鼠模型中研究了GEPs对免疫调节活性、肠道屏障功能和肠道微生物群调节的影响。
天麻多糖减轻了CTX诱导的胸腺和脾脏器官指数下降，并促进了血清中免疫相关细胞因子和免疫球蛋白的分泌。它们还改善了肠道病理学，并通过提高肠道紧密连接蛋白occludin和ZO-1的表达恢复了肠道屏障功能。此外，GEPs恢复了肠道微生物群的组成和丰度，并增加了结肠中短链脂肪酸（SCFA）的含量。产生SCFA的细菌（毛螺菌科、普雷沃氏菌科和拟杆菌科）的丰度也增加了。
天麻多糖可以有效减轻CTX处理小鼠的免疫抑制，并调节肠道屏障完整性和肠道微生物群结构。它们可作为开发肠道健康功能食品的成分。©2023化学工业协会。
