基于 E6、E7 和 L1 基因的中国中部地区人乳头瘤病毒 18 型遗传变异。

Genetic variability of human papillomavirus type 18 based on E6, E7 and L1 genes in central China.

机构信息

Department of Laboratory Medicine, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, 434020, China.

出版信息

Virol J. 2024 Jul 5;21(1):152. doi: 10.1186/s12985-024-02424-9.

DOI:10.1186/s12985-024-02424-9

PMID:38970084

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11227198/

Abstract

BACKGROUND

High-risk human papillomavirus (HR-HPV) infection is an important factor for the development of cervical cancer. HPV18 is the second most common HR-HPV after HPV16.

METHODS

In this study, MEGA11 software was used to analyze the variation and phylogenetic tree of HPV18 E6-E7 and L1 genes. The selective pressure to E6, E7 and L1 genes was estimated using pamlX. In addition, the B cell epitopes of L1 amino acid sequences and T cell epitopes of E6-E7 amino acid sequences in HPV18 were predicted by ABCpred server and IEDB website, respectively.

RESULTS

A total of 9 single nucleotide variants were found in E6-E7 sequences, of which 2 were nonsynonymous variants and 7 were synonymous variants. Twenty single nucleotide variants were identified in L1 sequence, including 11 nonsynonymous variants and 9 synonymous variants. Phylogenetic analysis showed that E6-E7 and L1 sequences were all distributed in A lineage. In HPV18 E6, E7 and L1 sequences, no positively selected site was found. The nonconservative substitution R545C in L1 affected hypothetical B cell epitope. Two nonconservative substitutions, S82A in E6, and R53Q in E7, impacted multiple hypothetical T cell epitopes.

CONCLUSION

The sequence variation data of HPV18 may lay a foundation for the virus diagnosis, further study of cervical cancer and vaccine design in central China.

摘要

背景

高危型人乳头瘤病毒（HR-HPV）感染是宫颈癌发展的重要因素。HPV18 是继 HPV16 之后第二常见的 HR-HPV。

方法

本研究使用 MEGA11 软件分析 HPV18 E6-E7 和 L1 基因的变异和系统进化树。使用 pamlX 估计 E6、E7 和 L1 基因的选择压力。此外，通过 ABCpred 服务器和 IEDB 网站分别预测 HPV18 L1 氨基酸序列的 B 细胞表位和 E6-E7 氨基酸序列的 T 细胞表位。

结果

E6-E7 序列中发现了 9 个单核苷酸变异，其中 2 个为非同义变异，7 个为同义变异。L1 序列中鉴定出 20 个单核苷酸变异，包括 11 个非同义变异和 9 个同义变异。系统进化分析显示，E6-E7 和 L1 序列均分布在 A 谱系中。HPV18 E6、E7 和 L1 序列中未发现正选择位点。L1 中的非保守性取代 R545C 影响假设的 B 细胞表位。E6 中的 S82A 和 E7 中的 R53Q 两个非保守性取代影响多个假设的 T 细胞表位。