Department of Biotechnology, Pharmacy School, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Sci Rep. 2023 Feb 8;13(1):2273. doi: 10.1038/s41598-023-29540-w.
The role of magnesium sulfate (MgSO) administration to prevent diabetic nephropathy (DN) by reducing insulin resistance (IR) and the relationship of this action with gender and the expression of NOX4 and ICAM1 genes in the parents and their offspring were studied. Males and females rat, and their pups were used. Type 2 diabetes induced by high-fat diet (HFD) administration and a low dose of streptozotocin. Animals were divided into the: non-treated diabetic (DC), the diabetic group received insulin (Ins), and the diabetic group received MgSO. Two groups of parents received just a normal diet (NDC). Following each set of parents for 16 weeks and their pups for 4 months, while eating normally. We assessed the amount of water consumed, urine volume, and blood glucose level. The levels of glucose, albumin, and creatinine in the urine were also measured, as well as the amounts of sodium, albumin, and creatinine in the serum. Calculations were made for glomerular filtration rate (GFR) and the excretion rates of Na and glucose fractions (FE Na and FE G, respectively). The hyperinsulinemic-euglycemic clamp was done. NOX4 and ICAM1 gene expressions in the kidney were also measured. MgSO or insulin therapy decreased blood glucose, IR, and improved GFR, FE Na, and FE G in both parents and their offspring compared to D group. MgSO improved NOX4 and ICAM1 gene expressions in the parents and their offspring compared to D group. Our results indicated that MgSO could reduce blood glucose levels and insulin resistance, and it could improve kidney function.
研究了硫酸镁（MgSO）通过降低胰岛素抵抗（IR）来预防糖尿病肾病（DN）的作用，以及这种作用与性别以及父母及其后代中 NOX4 和 ICAM1 基因表达的关系。使用雄性和雌性大鼠及其幼崽。通过高脂肪饮食（HFD）给药和低剂量链脲佐菌素诱导 2 型糖尿病。动物分为：未治疗的糖尿病（DC）、接受胰岛素（Ins）的糖尿病组和接受 MgSO 的糖尿病组。两组父母仅接受正常饮食（NDC）。在正常饮食下，分别对每组父母喂养 16 周，对其幼崽喂养 4 个月。评估水的摄入量、尿量和血糖水平。还测量了尿液中葡萄糖、白蛋白和肌酐的水平，以及血清中钠、白蛋白和肌酐的量。计算肾小球滤过率（GFR）和 Na 和葡萄糖分数的排泄率（FE Na 和 FE G）。进行高胰岛素-正常血糖钳夹。还测量了肾脏中 NOX4 和 ICAM1 基因的表达。与 D 组相比，MgSO 或胰岛素治疗降低了父母及其后代的血糖、IR，并改善了 GFR、FE Na 和 FE G。MgSO 改善了父母及其后代的 NOX4 和 ICAM1 基因表达。我们的结果表明，MgSO 可以降低血糖水平和胰岛素抵抗，并改善肾功能。
