Liu Fang, Luo Yingzhi, Chen Huan, Xu Shengjing, Zhang Dongyan, Sang Hong, Xu Chenjie, Zhang Min
Department of Dermatology, Jinling Hospital, School of Medicine, Nanjing University Nanjing China
Department of Dermatology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine Hangzhou 310006 China.
Nanoscale Adv. 2022 Dec 23;5(3):927-933. doi: 10.1039/d2na00604a. eCollection 2023 Jan 31.
Microneedle technology can effectively suppress the formation of hypertrophic scarring in both animals and humans. Our previous research has revealed that this is due to the physical contact inhibition effect by using microneedles made of liquid-crystal polymers as the model device. One important factor we didn't study is the influence of the fabrication materials of microneedles. Therefore, this article examines this key point on a rabbit ear hypertrophic scar model. We monitor the thickness of the scars, and the expression of α-SMA and Ki-67 protein, and TGF-β1 mRNA in a period of 42 days. Among microneedles made of 6 polymeric materials and stainless steel, polymethylmethacrylate microneedles present superiority in all aspects including the reduction of tissue fibrosis, and the expression of α-SMA, Ki-67 protein and TGF-β1 mRNA. On the other hand, polycarbonates, polyurethane, and polylactic--glycolic acid microneedles could suppress three biomarker expressions.
微针技术能够有效抑制动物和人类肥厚性瘢痕的形成。我们之前的研究表明，这是由于以液晶聚合物制成的微针作为模型装置产生的物理接触抑制作用。我们尚未研究的一个重要因素是微针制造材料的影响。因此，本文在兔耳肥厚性瘢痕模型上考察了这一关键点。我们在42天的时间内监测瘢痕厚度、α-SMA和Ki-67蛋白的表达以及TGF-β1 mRNA的表达。在由6种聚合材料和不锈钢制成的微针中，聚甲基丙烯酸甲酯微针在减少组织纤维化以及α-SMA、Ki-67蛋白和TGF-β1 mRNA的表达等所有方面均表现出优越性。另一方面，聚碳酸酯、聚氨酯和聚乳酸-乙醇酸微针能够抑制三种生物标志物的表达。
