Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Department Veterinary and Biosciences, Faculty Veterinary Medicine, University of Ghent, Ghent, Belgium.
Euro Surveill. 2023 Feb;28(6). doi: 10.2807/1560-7917.ES.2023.28.6.2200496.
IntroductionEnterococci harbouring genes encoding resistance to florfenicol and the oxazolidinone antimicrobial linezolid have emerged among food-producing animals and meat thereof, but few studies have analysed their occurrence in raw meat-based diets (RMBDs) for pets.AimWe aimed to examine how far RMBDs may represent a source of bacteria with oxazolidinone resistance genes.MethodsFifty-nine samples of different types of RMBDs from 10 suppliers (three based in Germany, seven in Switzerland) were screened for florfenicol-resistant Gram-positive bacteria using a selective culture medium. Isolates were phenotypically and genotypically characterised.ResultsA total of 27 , , and isolates were obtained from 24 of the 59 samples. The , , and genes were identified in 24/27, 6/27 and 5/27 isolates, respectively. Chloramphenicol and linezolid minimum inhibitory concentrations (MICs) ranged from 24.0 mg/L-256.0 mg/L, and 1.5 mg/L-8.0 mg/L, respectively. According to the Clinical and Laboratory Standards Institute (CLSI) breakpoints, 26 of 27 isolates were resistant to chloramphenicol (MICs ≥ 32 mg/L), and two were resistant to linezolid (MICs ≥ 8 mg/L). Multilocus sequence typing analysis of the 17 isolates identified 10 different sequence types (ST)s, with ST593 (n = 4 isolates) and ST207 (n = 2 isolates) occurring more than once, and two novel STs (n = 2 isolates). isolates belonged to four different STs (168, 264, 822, and 1846).ConclusionThe high occurrence in our sample of Gram-positive bacteria harbouring genes encoding resistance to the critical antimicrobial linezolid is of concern since such bacteria may spread from companion animals to humans upon close contact between pets and their owners.
介绍肠球菌携带编码对氟苯尼考和恶唑烷酮类抗菌药物利奈唑胺耐药的基因已经在食用动物及其肉类中出现，但很少有研究分析过它们在宠物用生肉基础日粮（RMBD）中的存在情况。
目的我们旨在研究 RMBD 可能在多大程度上成为具有恶唑烷酮类耐药基因的细菌的来源。
方法使用选择性培养基筛选来自 10 个供应商（德国 3 个，瑞士 7 个）的 59 种不同类型 RMBD 的不同类型的 RMBD 中对氟苯尼考耐药的革兰氏阳性菌。对分离株进行表型和基因型鉴定。
结果从 59 个样本中的 24 个样本中获得了总共 27 株、株和株分离株。在 27 株分离株中分别鉴定出了、和基因，在 6 株、5 株和 27 株分离株中分别鉴定出了和基因。氯霉素和利奈唑胺的最小抑菌浓度（MIC）范围分别为 24.0 毫克/升至 256.0 毫克/升和 1.5 毫克/升至 8.0 毫克/升。根据临床和实验室标准研究所（CLSI）的折点，27 株分离株中有 26 株对氯霉素耐药（MICs≥32 毫克/升），有 2 株对利奈唑胺耐药（MICs≥8 毫克/升）。17 株分离株的多位点序列分型分析鉴定出 10 种不同的序列类型（ST），其中 ST593（4 株分离株）和 ST207（2 株分离株）多次出现，还有 2 种新型 ST（2 株分离株）。株属于四个不同的 ST（168、264、822 和 1846）。
结论在我们的样本中，革兰氏阳性菌携带编码对关键抗菌药物利奈唑胺耐药的基因的高发生率令人担忧，因为这些细菌可能会在宠物和主人之间密切接触时从伴侣动物传播到人类。
