Gião Joana, Leão Célia, Albuquerque Teresa, Clemente Lurdes, Amaro Ana
Laboratory of Bacteriology and Mycology, INIAV-National Institute of Agrarian and Veterinary Research, 2780-157 Oeiras, Portugal.
Department of Veterinary Medicine, University of Évora, 7002-554 Évora, Portugal.
Antibiotics (Basel). 2022 May 3;11(5):615. doi: 10.3390/antibiotics11050615.
Enterococci are part of the commensal gut microbiota of mammals, with and being the most clinically relevant species. This study assesses the prevalence and diversity of enterococcal species in cattle ( = 201) and pig ( = 249) cecal samples collected in 2017. Antimicrobial susceptibility profiles of ( = 48) and ( = 84) were assessed by agar and microdilution methods. Resistance genes were screened through PCR and nine strains were analyzed by Whole Genome Sequencing. A wide range of enterococci species was found colonizing the intestines of pigs and cattle. Overall, the prevalence of resistance to critically important antibiotics was low (except for erythromycin), and no glycopeptide-resistant isolates were identified. Two daptomycin-resistant ST58 and ST93 were found. Linezolid-resistant strains of ( = 3) and ( = 1) were detected. Moreover, oxazolidinone resistance determinants ( = 8) and ( = 2) were found in (ST16, ST58, ST207, ST474, ST1178) and (ST22, ST2138). Multiple variants of were found in different genetic contexts, either in the chromosome or plasmids. We highlight the importance of animals as reservoirs of resistance genes to critically important antibiotics.
肠球菌是哺乳动物肠道共生微生物群的一部分，其中粪肠球菌和屎肠球菌是临床上最相关的物种。本研究评估了2017年采集的牛（n = 201）和猪（n = 249）盲肠样本中肠球菌物种的流行情况和多样性。通过琼脂法和微量稀释法评估了48株粪肠球菌和84株屎肠球菌的抗菌药敏谱。通过PCR筛选耐药基因，并对9株菌株进行全基因组测序分析。在猪和牛的肠道中发现了多种肠球菌物种。总体而言，对极其重要抗生素的耐药率较低（红霉素除外），未鉴定出耐糖肽类分离株。发现了两株耐达托霉素的屎肠球菌ST58和ST93。检测到3株耐利奈唑胺的粪肠球菌和1株耐利奈唑胺的屎肠球菌。此外，在粪肠球菌（ST16、ST58、ST207、ST474、ST1178）和屎肠球菌（ST22、ST2138）中发现了恶唑烷酮耐药决定簇cfr（n = 8）和optrA（n = 2）。在不同的遗传背景下，即在染色体或质粒中，发现了多种cfr变体。我们强调了动物作为极其重要抗生素耐药基因储存库的重要性。
