利用纳米孔宏基因组下一代测序技术快速检测感染性葡萄膜炎病原体：一项初步研究。
Rapid Pathogen Detection in Infectious Uveitis Using Nanopore Metagenomic Next-Generation Sequencing: A Preliminary Study.
机构信息
Department of Ophthalmology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Ocul Immunol Inflamm. 2024 May;32(4):463-469. doi: 10.1080/09273948.2023.2173243. Epub 2023 Feb 9.
PURPOSE
We establish an accurate and rapid diagnostic method for pathogen detection in infectious uveitis using nanopore metagenomic next-generation sequencing (NGS).
METHODS
In eight patients with suspected infectious uveitis, we prospectively compared the accuracy and time taken for pathogen identification between conventional diagnostic methods, such as cultures and PCR, and nanopore metagenomic NGS.
RESULTS
All causative pathogens were identified using nanopore sequencing, while only five of the eight patients were confirmed positive for a specific pathogen using conventional methods. The overall sample-to-answer turnaround time of nanopore sequencing was much shorter than that of conventional methods in the bacterial and fungal infection (mean, 17 h vs. 4d, respectively; = .028). The pathogens could be detected even when the quantity or quality of DNA was not optimal.
CONCLUSION
Nanopore metagenomic NGS is a promising diagnostic tool that can rapidly and accurately identify the causative pathogen in infectious uveitis.
目的
我们使用纳米孔宏基因组下一代测序（NGS）建立一种用于检测传染性眼内炎病原体的准确、快速的诊断方法。
方法
在 8 名疑似传染性眼内炎患者中，我们前瞻性地比较了传统诊断方法（如培养和 PCR）和纳米孔宏基因组 NGS 在病原体鉴定方面的准确性和所需时间。
结果
所有病原体均通过纳米孔测序确定，而使用传统方法仅确认 8 名患者中的 5 名患有特定病原体。纳米孔测序的总体样本到答案周转时间明显短于传统方法在细菌和真菌感染中的时间（分别为平均 17 小时与 4 天；= 0.028）。即使 DNA 的数量或质量不理想，也可以检测到病原体。
结论
纳米孔宏基因组 NGS 是一种很有前途的诊断工具，可快速准确地确定传染性眼内炎的病原体。
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