Ali Maqsood, Farwa Ume, Park Seong-Su, Kim Yong-Sik, Lee Byong-Taek
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, South Korea.
Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, South Korea.
Biomater Adv. 2023 Apr;147:213326. doi: 10.1016/j.bioadv.2023.213326. Epub 2023 Feb 3.
In this study, we investigated the physico-biological and in-vivo evaluation of irisin loaded 45S5 bioglass bone graft for enhancing osteoblastic differentiation and bone regeneration in rat femur head defect model. Highly porous structure was obtained in the bioglass by burn-out process with varying the concentration of poly (methyl methacrylate) (PMMA) spheres. 10 % polyvinyl alcohol (PVA) was used as a binder for the sustain releasing of irisin on porous bioglass. Different concentrations of irisin were loaded on the selected bioglass samples and these were further evaluated for the biocompatibility and osteoblastic differentiation properties. The in vitro results demonstrated not only its biocompatibility but also that it stimulated pre-osteoblast differentiation. The in vivo data showed new bone formation as well as expression of osteogenic proteins like alkaline phosphatase (ALP), Runt-related transcription factor 2 (Runx-2), osteopontin (OPN), and collagen-1 (Col-1). Our results support the use of irisin loaded bioglass for the use of early bone regeneration.
在本研究中，我们对负载鸢尾素的45S5生物活性玻璃骨移植材料进行了物理生物学和体内评估，以增强大鼠股骨头缺损模型中的成骨细胞分化和骨再生。通过改变聚甲基丙烯酸甲酯（PMMA）微球的浓度，采用烧蚀工艺在生物活性玻璃中获得了高度多孔的结构。使用10%的聚乙烯醇（PVA）作为载体，使鸢尾素在多孔生物活性玻璃上持续释放。将不同浓度的鸢尾素负载到选定的生物活性玻璃样品上，并进一步评估其生物相容性和成骨细胞分化特性。体外实验结果不仅证明了其生物相容性，还表明它能刺激前成骨细胞分化。体内数据显示有新骨形成，以及碱性磷酸酶（ALP）、Runx相关转录因子2（Runx-2）、骨桥蛋白（OPN）和胶原蛋白-1（Col-1）等成骨蛋白的表达。我们的结果支持使用负载鸢尾素的生物活性玻璃促进早期骨再生。
