Zhu Jiang, Xu Teng, Song Dong-Hui, Wu Sen-Bin
Department of Stomatology, Affiliated Hospital of Nantong University. Nantong 226000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Oct;31(5):517-522.
To explore the relationship between combined detection of tissue active protein kinase C receptor 1 (RACK1) and M2/M1 and the prognosis of oral squamous cell carcinoma (OSCC).
129 OSCC patients admitted to the Affiliated Hospital of Nantong University were selected as the research subjects from February 2017 to May 2018. RACK1 and M2/M1 in cancer tissues and adjacent tissues of patients were detected and compared. The relationship between RACK1 expression, M2/M1 and clinicopathological factors, and relationship between RACK1 expression, M2/M1 and prognosis in cancer tissue were analyzed. SPSS 18.0 software package was used for data analysis.
The positive expression rate of RACK1 and the value of M2/M1 in cancer tissues were significantly higher than those in adjacent tissues (P<0.05). The positive expression rate of RACK1 in cancer tissues of patients with stage Ⅲ-Ⅳ, cervical lymph node metastasis, and vascular involvement were significantly higher than those with stage Ⅰ-Ⅱ, no lymph node metastasis, and no vascular involvement(P<0.05). M2/M1 values in cancer tissues of patients with stage Ⅲ-Ⅳ, poor differentiation, and cervical lymph node metastasis were significantly higher than those with stage Ⅰ-Ⅱ, moderate differentiation, and no cervical lymph node metastasis(P<0.05). Cox regression analysis showed that the stage of tumor, cervical lymph node metastasis, positive expression rate of RACK1, and M2/M1 were independent prognostic factors affecting overall survival (P<0.05). ROC curve showed that AUC of cancer tissue RACK1, M2/M1 and combination of the two to predict the prognosis of OSCC patients were 0.743, 0.718 and 0.875, respectively. The survival rate of patients with positive expression of RACK1 was 62.24%, and the survival rate of patients with negative expression of RACK1 was 92.31%. The overall survival rate of patients with positive expression of RACK1 and patients with negative expression were compared, and the difference was statistically significant(P<0.05). The survival rate of patients with M2/M1≥2.06 was 61.70%, the survival rate of patients with M2/M1<2.06 was 88.24%, and the overall survival curve of patients with M2/M1≥2.06 was compared with that of patients with M2/M1<2.06, and the difference was statistically significant(P<0.05).
The expression of RACK1 and M2/M1 is abnormally high in cancer tissues of OSCC patients. The expression of RACK1 and M2/M1 is related to the patient's pathology and prognosis. The combination of the two has certain efficacy in predicting the prognosis of OSCC patients.
探讨组织活性蛋白激酶C受体1（RACK1）与M2/M1联合检测与口腔鳞状细胞癌（OSCC）预后的关系。
选取2017年2月至2018年5月在南通大学附属医院收治的129例OSCC患者作为研究对象。检测并比较患者癌组织及癌旁组织中RACK1和M2/M1的表达情况。分析癌组织中RACK1表达、M2/M1与临床病理因素的关系，以及RACK1表达、M2/M1与预后的关系。采用SPSS 18.0软件包进行数据分析。
癌组织中RACK1阳性表达率及M2/M1值均显著高于癌旁组织（P<0.05）。Ⅲ-Ⅳ期、有颈部淋巴结转移、有血管侵犯患者癌组织中RACK1阳性表达率显著高于Ⅰ-Ⅱ期、无淋巴结转移、无血管侵犯患者（P<0.05）。Ⅲ-Ⅳ期、低分化、有颈部淋巴结转移患者癌组织中M2/M1值显著高于Ⅰ-Ⅱ期、中分化、无颈部淋巴结转移患者（P<0.05）。Cox回归分析显示，肿瘤分期、颈部淋巴结转移、RACK1阳性表达率及M2/M1是影响总生存的独立预后因素（P<0.05）。ROC曲线显示，癌组织RACK1、M2/M1及两者联合预测OSCC患者预后的AUC分别为0.743、0.718和0.875。RACK1阳性表达患者生存率为62.24%，RACK1阴性表达患者生存率为92.31%。比较RACK1阳性表达患者与阴性表达患者的总生存率，差异有统计学意义（P<0.05）。M2/M1≥2.06患者生存率为61.70%，M2/M1<2.06患者生存率为88.24%，比较M2/M1≥2.06患者与M2/M1<2.06患者的总生存曲线，差异有统计学意义（P<0.05）。
OSCC患者癌组织中RACK1及M2/M1表达异常增高。RACK1及M2/M1表达与患者病理及预后相关。两者联合对OSCC患者预后预测有一定效能。
