Javed Saman, Munir Aneela, Javed Ghulam Ayesha, Latif Zakia, Javed Sehrish, Arshad Najma
Institute of Zoology, University of the Punjab, Lahore 54590, Pakistan.
School of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.
Lett Appl Microbiol. 2023 Mar 1;76(3). doi: 10.1093/lambio/ovad024.
Probiotics are known to possess strain- and species-specific functional properties, of which hypocholesteremia is of major interest. Bile salt hydrolase (BSH) activity is one of the key mechanisms involved in the hypocholesterolemic effect. The study was designed to genetically characterize probiotics obtained from human milk on the basis of simple sequence repeat (SSR), isolate potent hypocholesterolemic strains, and detect BSH activity, deconjugation of bile salts, and bsh polymorphism. This study, for the first time, linked genetic diversity with cholesterol reduction potential and proved the presence of conserved bsh of Levilactobacillus brevis in genetically diverse species. The strains displayed 2.78%-42.23% cholesterol reduction, which was not influenced by prebiotics. In this study, data obtained from SSR markers indicated 93.3% diversity, and based on cluster analysis, they were distributed into XI clades; out of five potent cholesterol-reducing strains, three belonged to clade I. The strains could deconjugate both sodium glycocholate and sodium taurocholate, but we preferred using sodium glycocholate. The variation in cholesterol reduction potential and BSH activity pointed toward the presence of more than one bsh in the strains. Weissella confusa MW051433 displayed highest cholesterol reduction (42.23%) and specific BSH activity (2.64 U ml -1). Search for other bsh and in vivo assessments of cholesterol reduction by W. confusa MW051433 have been proposed.
已知益生菌具有菌株和物种特异性的功能特性，其中降胆固醇作用备受关注。胆汁盐水解酶（BSH）活性是降胆固醇作用涉及的关键机制之一。本研究旨在基于简单序列重复（SSR）对从人乳中获得的益生菌进行基因特征分析，分离出有效的降胆固醇菌株，并检测BSH活性、胆汁盐的去结合作用以及bsh多态性。本研究首次将遗传多样性与胆固醇降低潜力联系起来，并证明了在遗传多样的物种中存在短乳杆菌保守的bsh。这些菌株的胆固醇降低率为2.78%-42.23%，不受益生元的影响。在本研究中，从SSR标记获得的数据表明多样性为93.3%，基于聚类分析，它们被分为11个进化枝；在5株有效的降胆固醇菌株中，有3株属于进化枝I。这些菌株可以使甘氨胆酸钠和牛磺胆酸钠去结合，但我们更倾向于使用甘氨胆酸钠。胆固醇降低潜力和BSH活性的差异表明菌株中存在不止一种bsh。困惑魏斯氏菌MW051433表现出最高的胆固醇降低率（42.23%）和特定的BSH活性（2.64 U ml-1）。已提议寻找其他bsh并对困惑魏斯氏菌MW051433的胆固醇降低进行体内评估。
