Cheng Yizhuang, Qin Miao, Li Pan, Yang Liangbao
Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences Hefei 230031 China
University of Science & Technology of China Hefei 230026 Anhui China.
RSC Adv. 2023 Feb 3;13(7):4584-4589. doi: 10.1039/d2ra07216e. eCollection 2023 Jan 31.
In recent years, marine biotoxins have posed a great threat to fishermen, human security and military prevention and control due to their diverse, complex, toxic and widespread nature, and the development of rapid and sensitive methods is essential. Surface-enhanced Raman spectroscopy (SERS) is a promising technique for the rapid and sensitive detection of marine biotoxins due to its advantages of rapid, high sensitivity, and fingerprinting information. However, the complex structure of toxin molecules, small Raman scattering cross-section and low affinity to conventional substrates make it difficult to achieve direct and sensitive SERS detection. Here, we generate a large number of active hotspot structures by constructing monolayer nanoparticle films with high density hotspots, which have good target molecules that can actively access the hotspot structures using nanocapillaries. In addition, the efficient and stable signal can be achieved during dynamic detection, increasing the practicality and operability of the method. This versatile SERS method achieves highly sensitive detection of marine biotoxins GTX and NOD, providing good prospects for convenient, rapid and sensitive SERS detection of marine biotoxins.
近年来，海洋生物毒素因其多样、复杂、有毒且广泛存在的特性，对渔民、人类安全和军事防控构成了巨大威胁，因此开发快速灵敏的检测方法至关重要。表面增强拉曼光谱（SERS）由于具有快速、高灵敏度和指纹信息等优点，是一种用于快速灵敏检测海洋生物毒素的有前途的技术。然而，毒素分子结构复杂、拉曼散射截面小以及对传统基底的亲和力低，使得难以实现直接且灵敏的SERS检测。在此，我们通过构建具有高密度热点的单层纳米颗粒薄膜来生成大量活性热点结构，这些结构具有良好的目标分子，能够利用纳米毛细管主动进入热点结构。此外，在动态检测过程中可以实现高效稳定的信号，提高了该方法的实用性和可操作性。这种通用的SERS方法实现了对海洋生物毒素GTX和NOD的高灵敏度检测，为方便、快速、灵敏地进行海洋生物毒素的SERS检测提供了良好的前景。
