Wang Yingcheng, Dang Ning, Feng Kai, Wang Junbang, Jin Xin, Yao Shiting, Wang Linlin, Gu Songsong, Zheng Hua, Lu Guangxin, Deng Ye
Collage of Agriculture and Animal Husbandry, Qinghai University, Xining, China.
CAS Key Laboratory for Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences (CAS), Beijing, China.
Front Microbiol. 2023 Jan 25;14:1109128. doi: 10.3389/fmicb.2023.1109128. eCollection 2023.
Associations between grasses and soil microorganisms can strongly influence plant community structures. However, the associations between grass productivity and diversity and soil microbes, as well as the patterns of co-occurrence between grass and microbes remain unclear. Here, we surveyed grass productivity and diversity, determined soil physicochemical, and sequenced soil archaea, bacteria and fungi by metabarcoding technology at 16 alpine grasslands. Using the Distance-decay relationship, Inter-Domain Ecological Network (IDEN), and Mantel tests, we investigated the relationship between grass productivity, diversity and microbial diversity, and the patterns of co-occurrence between grass and microbial inter-domain network in alpine grassland. We found the archaea richness, bacteria richness and Shannon, and fungi α-diversity were significantly negatively correlation with grass diversity, but archaea and bacteria diversity were positively correlation with grass productivity. Moreover, an increase in microbial β-diversity was observed along with increased discrepancy in grass diversity and productivity and soil variables. Variance partitioning analysis suggested that the contribution of grass productivity on microbial community was higher than that of soil variables and grass diversity, which implies that microbial community was more related to grass productivity. Inter-Domain Ecological Network showed that the grass species formed complex and stable ecological networks with some bacterial, archaeal, and fungal species, and the grass-fungal ecological networks showed the highest robustness, which indicated that soil fungi could better co-coexist with aboveground grass in alpine grasslands. Besides, the connectivity degrees of the grass-microbial network were significantly positively correlated with grass productivity, suggesting that the coexistence pattern of grasses and microbes had a positive feedback effect on the grass productivity. The results are important for establishing the regulatory mechanisms between plants and microorganisms in alpine grassland ecosystems.
禾本科植物与土壤微生物之间的关联会强烈影响植物群落结构。然而，禾本科植物生产力和多样性与土壤微生物之间的关联，以及禾本科植物与微生物之间的共现模式仍不清楚。在此，我们在16个高寒草原调查了禾本科植物生产力和多样性，测定了土壤理化性质，并通过代谢条形码技术对土壤古菌、细菌和真菌进行了测序。利用距离衰减关系、域间生态网络（IDEN）和Mantel检验，我们研究了高寒草原中禾本科植物生产力、多样性与微生物多样性之间的关系，以及禾本科植物与微生物域间网络之间的共现模式。我们发现古菌丰富度、细菌丰富度和香农指数，以及真菌α多样性与禾本科植物多样性显著负相关，但古菌和细菌多样性与禾本科植物生产力正相关。此外，随着禾本科植物多样性、生产力以及土壤变量差异的增加，微生物β多样性也有所增加。方差分解分析表明，禾本科植物生产力对微生物群落的贡献高于土壤变量和禾本科植物多样性，这意味着微生物群落与禾本科植物生产力的关系更为密切。域间生态网络表明，禾本科植物物种与一些细菌、古菌和真菌物种形成了复杂而稳定的生态网络，禾本科植物-真菌生态网络表现出最高的稳健性，这表明土壤真菌能够更好地与高寒草原地上的禾本科植物共存。此外，禾本科植物-微生物网络的连接度与禾本科植物生产力显著正相关，表明禾本科植物与微生物的共存模式对禾本科植物生产力具有正反馈作用。这些结果对于建立高寒草原生态系统中植物与微生物之间的调控机制具有重要意义。
