Yang Hao, Ma Yansong, Xie Xianju, Wang Hongmei, Li Xiaowei, Fang Dongyu, Bai Yuxing
Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China.
Front Microbiol. 2023 Jan 24;14:1084850. doi: 10.3389/fmicb.2023.1084850. eCollection 2023.
White spot lesions (WSLs) are common enamel infectious diseases in fixed orthodontic treatment, which might attribute to the dysbiosis of oral microbiome. However, the correlation of with oral bacteriome in WSLs still remains unrevealed. This study investigated the carriage of and how it shaped the bacterial community in disease or healthy supragingival plaque, to explore the potential role of interkingdom interaction in orthodontic WSLs. In this study, 31 patients with WSLs (WSLs) and 23 healthy patients (Health) undergoing fixed orthodontic treatment were enrolled. The supragingival microbiota in both groups were determined using 16S rRNA gene sequencing. Colonization and abundance of in the plaque were determined culture-dependent and -independent methods. Among WSLs patients, the correlation of and bacteriome was analyzed under QIIME2-based bioinformatics and Spearman's correlation coefficient. The raw reads were deposited into the NCBI Sequence Read Archive (SRA) database (Accession Number: SRP404186). Significant differences in microbial diversity as well as composition were observed between WSLs and Health groups. remarkably enriched in the WSLs group, while and significantly enriched in the Health group. In addition, 45% of WSLs patients were carriers but none in patients without WSLs. Among all WSLs patients, beta diversity and microbial composition were distinguished between carriers and non-carriers. In carriers, and significantly enriched whereas significantly depleted. The abundance of was positively associated with bacteria such as , while the negative correlation was detected between and several bacteria such as and Our study elucidated the distinguished supragingival plaque microbiome between orthodontic patients with and without WSLs. frequently existed and enriched in orthodontic derived WSLs. The carriage of shape plaque bacterial community in demineralized lesions and might play roles in WSLs pathogenesis.
白斑病变（WSLs）是固定正畸治疗中常见的牙釉质感染性疾病，可能归因于口腔微生物群的失调。然而，WSLs与口腔细菌群落的相关性仍未明确。本研究调查了[具体内容缺失]的携带情况及其如何塑造疾病或健康龈上菌斑中的细菌群落，以探讨跨界相互作用在正畸WSLs中的潜在作用。在本研究中，纳入了31例接受固定正畸治疗的WSLs患者（WSLs组）和23例健康患者（健康组）。使用16S rRNA基因测序确定两组的龈上微生物群。通过依赖培养和不依赖培养的方法确定菌斑中[具体内容缺失]的定植和丰度。在WSLs患者中，基于QIIME2的生物信息学和Spearman相关系数分析[具体内容缺失]与细菌群落的相关性。原始读数存入NCBI序列读数存档（SRA）数据库（登录号：SRP404186）。观察到WSLs组和健康组之间微生物多样性和组成存在显著差异。[具体内容缺失]在WSLs组中显著富集，而[具体内容缺失]和[具体内容缺失]在健康组中显著富集。此外，45%的WSLs患者是[具体内容缺失]携带者，而无WSLs的患者中没有携带者。在所有WSLs患者中，[具体内容缺失]携带者和非携带者之间的β多样性和微生物组成存在差异。在[具体内容缺失]携带者中，[具体内容缺失]和[具体内容缺失]显著富集，而[具体内容缺失]显著减少。[具体内容缺失]的丰度与[具体内容缺失]等细菌呈正相关，而与[具体内容缺失]和[具体内容缺失]等几种细菌呈负相关。我们的研究阐明了正畸有或无WSLs患者之间龈上菌斑微生物群的差异。[具体内容缺失]在正畸源性WSLs中经常存在并富集。[具体内容缺失]的携带影响脱矿病变中菌斑细菌群落并可能在WSLs发病机制中起作用。
