Migliaccio G, Migliaccio A R
Department of Haematology, Istituto Superiore di Sanità, Rome, Italy.
Br J Haematol. 1987 Oct;67(2):129-33. doi: 10.1111/j.1365-2141.1987.tb02315.x.
We describe culture conditions which enabled us to clone early human erythroid progenitors (BFU-E) in the absence of fetal bovine serum (FBS). Our medium, which is chemically fully defined, supports proliferation and differentiation of erythroid progenitors comparable to that in FBS-supplemented cultures. Furthermore, it allows cloning of BFU-E from all human haemopoietic tissues (adult marrow, embryonic liver and yolk sac) and adult or perinatal blood. This system should facilitate the investigation of human erythroid differentiation in vitro (e.g. cell-cell interaction, mechanism of action of haemopoietins and inhibitors, regulation of Hb synthesis) as well as the mechanisms underlying myeloproliferative disorders. As an example, we have found that recombinant Ep shows, in this culture system, the same dose response as Ep from conventional sources.
我们描述了一种培养条件，该条件使我们能够在无胎牛血清（FBS）的情况下克隆早期人类红系祖细胞（BFU-E）。我们的培养基化学成分完全明确，可支持红系祖细胞的增殖和分化，与补充了FBS的培养条件相当。此外，它还能从所有人类造血组织（成人骨髓、胚胎肝脏和卵黄囊）以及成人或围产期血液中克隆BFU-E。该系统应有助于体外研究人类红系分化（如细胞间相互作用、造血生长因子和抑制剂的作用机制、血红蛋白合成的调控）以及骨髓增殖性疾病的潜在机制。例如，我们发现重组Ep在该培养系统中表现出与传统来源的Ep相同的剂量反应。
