Braker Emily M
Vertebrate Zoology, University of Colorado Museum of Natural History, UCB 265, Boulder CO 80309, USA University of Colorado Museum of Natural History Boulder United States of America.
Zookeys. 2022 Dec 9;1134:185-210. doi: 10.3897/zookeys.1134.96103. eCollection 2022.
Fluid-preserved reptile and amphibian specimens are challenging to photograph with traditional methods due to their complex three-dimensional forms and reflective surfaces when removed from solution. An effective approach to counteract these issues involves combining focus stack photography with the use of a photo immersion tank. Imaging specimens beneath a layer of preservative fluid eliminates glare and risk of specimen desiccation, while focus stacking produces sharp detail through merging multiple photographs taken at successive focal steps to create a composite image with an extended depth of field. This paper describes the wet imaging components and focus stack photography workflow developed while conducting a large-scale digitization project for targeted reptile and amphibian specimens housed in the University of Colorado Museum of Natural History Herpetology Collection. This methodology can be implemented in other collections settings and adapted for use with fluid-preserved specimen types across the Tree of Life to generate high-quality, taxonomically informative images for use in documenting biodiversity, remote examination of fine traits, inclusion in publications, and educational applications.
由于保存在液体中的爬行动物和两栖动物标本从溶液中取出后具有复杂的三维形态和反光表面，使用传统方法对其进行拍摄具有挑战性。一种有效解决这些问题的方法是将聚焦堆叠摄影与使用照片浸泡池相结合。在一层保存液下方对标本进行成像可消除眩光和标本干燥的风险，而聚焦堆叠则通过合并在连续对焦步骤拍摄的多张照片来产生清晰的细节，从而创建具有扩展景深的合成图像。本文描述了在为科罗拉多大学自然历史博物馆爬虫学收藏中保存的目标爬行动物和两栖动物标本开展大规模数字化项目时所开发的湿成像组件和聚焦堆叠摄影工作流程。这种方法可以在其他收藏环境中实施，并适用于整个生命树中保存在液体中的标本类型，以生成高质量、具有分类学信息的图像，用于记录生物多样性、远程检查精细特征、纳入出版物以及教育应用。
