Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Prostaglandins Other Lipid Mediat. 2023 Apr;165:106716. doi: 10.1016/j.prostaglandins.2023.106716. Epub 2023 Feb 8.
The investigation of serum leptin, ghrelin, insulin, seratonin hormones, NO, total oxidant/antioxidant status and brain cannaboid 1 receptor protein and apoptotic cell levels in atorvastatin and Lactobacillus acidophilus administrated experimental hypercholesterolemia was aimed in the project.
In the study, 5 experimental groups were formed. Group 1 was fed with standard rat chow, and Group 2 was fed with 2% cholesterol added standard rat chow for 8 weeks. Group 3 was fed with 2% cholesterol feed and received atorvastatin (20 mg/kg/day) for the last 4 weeks. Group 4 was given L. acidophilus (2 ×10 cfu/kg/day). Group 5 was given atorvastatin and L. acidophilus probiotic in the last 4 weeks of the experiment period. After the experimental period, blood samples were taken from each rat. Rats were sacrificed and brain tissues were taken for analyzes. In sera samples, leptin, ghrelin, insulin, serotonin hormones and NO levels were measured with ELISA. In brain samples, cannabinoid 1 receptor proteins and apoptosis levels were measured by ELISA. Total oxidant and antioxidant levels were investigated with using Rel Assay Kits.
The addition of cholesterol to feeds increased the levels of serum cholesterol, insulin and leptin levels; on the other hand, reduced the levels of serotonin and ghrelin. In hypercholesterolemia, total oxidant and NO levels were increased, and total antioxidant levels were decreased.
The results showed that administrations of L. acidophilus and atorvastatin might be recommended for treatment of hypercholesterolemia.
本研究旨在探讨阿托伐他汀和嗜酸乳杆菌给药后实验性高胆固醇血症大鼠血清瘦素、胃饥饿素、胰岛素、血清素、一氧化氮（NO）、总氧化剂/抗氧化剂状态、脑大麻素 1 受体蛋白和凋亡细胞水平的变化。
在这项研究中，我们构建了 5 个实验组。第 1 组给予标准大鼠饲料，第 2 组给予 2%胆固醇添加的标准大鼠饲料 8 周。第 3 组给予 2%胆固醇饲料，并在最后 4 周给予阿托伐他汀（20mg/kg/天）。第 4 组给予嗜酸乳杆菌（2×10cfu/kg/天）。第 5 组在实验的最后 4 周给予阿托伐他汀和嗜酸乳杆菌益生菌。实验结束后，从每只大鼠取血。处死大鼠取脑进行分析。在血清样本中，采用 ELISA 法测定瘦素、胃饥饿素、胰岛素、血清素和 NO 水平。在脑样本中，采用 ELISA 法测定大麻素 1 受体蛋白和凋亡水平。采用 Rel 测定试剂盒测定总氧化剂和抗氧化剂水平。
饲料中添加胆固醇可升高血清胆固醇、胰岛素和瘦素水平，降低血清素和胃饥饿素水平。在高胆固醇血症中，总氧化剂和 NO 水平升高，总抗氧化剂水平降低。
研究结果表明，嗜酸乳杆菌和阿托伐他汀的联合使用可能有助于治疗高胆固醇血症。
