Kapar Fatma Semina, Ciftci Gülay
Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Atakum, 55200 Samsun, Turkey.
J Diabetes Metab Disord. 2020 Aug 27;19(2):907-914. doi: 10.1007/s40200-020-00578-1. eCollection 2020 Dec.
In this study, we tried to investigate the effects of curcumin and probiotics, given individually and in combination, to insulin, adipokines and nitric oxide changes and insulin resistance as experimental treatment of metabolic syndrome.
Five groups were formed in the study. Fructose (20%) was administered with drinking water for 8 weeks to develop metabolic syndrome. For treatment, curcumin (100 mg/kg/day) and (2 × 10 cfu/ml/day) were given individually or in combination for the last four weeks. At the end of the experiment; insulin, resistin, leptin, adipokines, apelin and nitric oxide levels were determined by ELISA test kits. Total cholesterol, triglyceride, glucose, albumin and total protein levels were determined by autoanalyzer.
The levels of apelin, resistin, glucose, total cholesterol and triglyceride increased significantly (P < 0.05) in the fructose added to drinking water groups whereas curcumin and probiotics given individually or together groups for treatment started to decrease and the nitric oxide level decreased significantly. Insulin resistance was found to be significantly higher in the group with metabolic syndrome and insulin resistance developed. In the curcumin and probiotics given group, it was determined that the insulin resistance score was lowered compared to the group only given fructose. The administration of probiotic and curcumin in rats with metabolic syndrome caused by fructose improves hormone levels and reduces insulin resistance.
These results showed that the addition of dietary curcumin as an antioxidant and probiotic could provide a natural alternative for the treatment of metabolic syndrome induced by fructose.
在本研究中，我们试图探究姜黄素和益生菌单独及联合使用对胰岛素、脂肪因子、一氧化氮变化以及胰岛素抵抗的影响，以此作为代谢综合征的实验性治疗方法。
本研究分为五组。给大鼠饮用含20%果糖的水8周以诱导代谢综合征。在最后四周进行治疗时，单独或联合给予姜黄素（100毫克/千克/天）和益生菌（2×10⁸ 菌落形成单位/毫升/天）。实验结束时，使用酶联免疫吸附测定试剂盒测定胰岛素、抵抗素、瘦素、脂肪因子、apelin和一氧化氮水平。通过自动分析仪测定总胆固醇、甘油三酯、葡萄糖、白蛋白和总蛋白水平。
饮用添加果糖的水的组中，apelin、抵抗素、葡萄糖、总胆固醇和甘油三酯水平显著升高（P<0.05），而单独或联合给予姜黄素和益生菌进行治疗的组这些指标开始下降，一氧化氮水平显著降低。发现代谢综合征及胰岛素抵抗组的胰岛素抵抗明显更高。在给予姜黄素和益生菌的组中，与仅给予果糖的组相比，胰岛素抵抗评分降低。给由果糖引起代谢综合征的大鼠施用益生菌和姜黄素可改善激素水平并降低胰岛素抵抗。
这些结果表明，添加作为抗氧化剂的膳食姜黄素和益生菌可为治疗果糖诱导的代谢综合征提供一种天然替代方法。
