Sahu Indra D, Lorigan Gary A
Natural Science Division, Campbellsville University, Campbellsville, KY, United States of America; Department of Chemistry and Biochemistry, Miami University, Oxford, OH, United States of America.
Department of Chemistry and Biochemistry, Miami University, Oxford, OH, United States of America.
Biochim Biophys Acta Biomembr. 2023 Apr;1865(4):184138. doi: 10.1016/j.bbamem.2023.184138. Epub 2023 Feb 9.
Biological membranes are essential in providing the stability of membrane proteins in a functional state. Functionally stable homogeneous sample is required for biophysical electron paramagnetic resonance (EPR) studies of membrane proteins for obtaining pertinent structural dynamics of the protein. Significant progresses have been made for the optimization of the suitable membrane environments required for biophysical EPR measurements. However, no universal membrane mimetic system is available that can solubilize all membrane proteins suitable for biophysical EPR studies while maintaining the functional integrity. Great efforts are needed to optimize the sample condition to obtain better EPR data quality of membrane proteins that can provide meaningful information on structural dynamics. In this mini-review, we will discuss important aspects of membrane mimetics for biophysical EPR measurements and current progress with some of the recent examples.
生物膜对于维持膜蛋白在功能状态下的稳定性至关重要。进行膜蛋白的生物物理电子顺磁共振（EPR）研究以获取蛋白质相关的结构动力学信息时，需要功能稳定的均一样品。在优化生物物理EPR测量所需的合适膜环境方面已经取得了显著进展。然而，目前尚无一种通用的膜模拟系统，能够溶解所有适合生物物理EPR研究的膜蛋白，同时保持其功能完整性。需要付出巨大努力来优化样品条件，以获得更好的膜蛋白EPR数据质量，从而提供有关结构动力学的有意义信息。在本综述中，我们将讨论用于生物物理EPR测量的膜模拟物的重要方面以及一些近期实例的研究进展。
