Department of Analytical Chemistry, University of Valencia, Dr Moliner 50, 46100, Burjassot, Valencia, Spain.
Institute of Material Science (ICMUV), University of Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, Spain.
Anal Chim Acta. 2023 Mar 15;1246:340887. doi: 10.1016/j.aca.2023.340887. Epub 2023 Jan 26.
In this work, metal-organic frameworks (MOFs) were used for the first time as solid-phase extraction (SPE) sorbents for the isolation of synthetic cannabinoids (SCs) from oral fluids and subsequently quantified by LC-fluorescence detection (FLD). In this context, different MOF families were synthesized and tested under SPE mode. UiO-66 was the family selected, being the amino functionalized (NH-UiO-66) the best candidate in terms of extraction performance. After the method optimization, several analytical parameters of interest were obtained, reaching limits of detection (LODs) as low as 0.6-0.8 μg L and precision values (expressed as RSD) lower than 10.6%. The developed method was successfully applied to the determination of 8 SCs in different oral fluids at three spiked levels with recoveries between 67 and 114%. This method claims to be a real alternative for screening purposes, being a cost-effective procedure due to the price of the sorbent (<0.5 €/cartridge) and its recyclability (up to 12 uses), among others good features.
在这项工作中，金属有机骨架（MOFs）首次被用作固相萃取（SPE）吸附剂，用于从口腔液中分离合成大麻素（SCs），并随后通过 LC-荧光检测（FLD）进行定量。在这种情况下，合成了不同的 MOF 家族并在 SPE 模式下进行了测试。UiO-66 是被选中的家族，在萃取性能方面，氨基功能化的（NH-UiO-66）是最佳候选物。在方法优化后，获得了几个感兴趣的分析参数，达到了低至 0.6-0.8μg/L 的检测限（LOD）和低于 10.6%的精密度值（表示为 RSD）。该方法成功地应用于三种不同水平的口腔液中 8 种 SCs 的测定，回收率在 67%至 114%之间。该方法声称是一种用于筛选目的的真正替代方法，由于吸附剂的价格（<0.5 欧元/个）及其可回收性（多达 12 次使用）等优点，是一种具有成本效益的程序。
