玉米素诱导香稻（Oryza sativa L.）幼苗中 2-乙酰-1-吡咯啉的生物合成。

Trans-Zeatin induce regulation the biosynthesis of 2-acetyl-1-pyrroline in fragrant rice (Oryza sativa L.) seedlings.

机构信息

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

Scientific Observing and Experimental Station of Crop Cultivation in South China, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, China.

出版信息

BMC Plant Biol. 2023 Feb 11;23(1):88. doi: 10.1186/s12870-023-04106-0.

BACKGROUND

In plants, cytokinin is activated into trans-zeatin to fight abiotic stresses. However, the mechanism of the effect of trans-zeatin on 2-acetyl-1-pyrroline (2-AP) biosynthesis in fragrant rice has yet to be studied. The present study was conducted to explore the effects of exogenous trans-zeatin on enzymes activities, genes expression, and precursors involved in 2-AP biosynthesis and 2-AP contents as well as the seedling quality of a fragrant rice cultivar viz., Meixiangzhan2. Four concentrations of trans-zeatin solutions at 20, 40, and 80 μmol L (ZT1, ZT2, and ZT3) were sprayed onto rice seedlings.

RESULTS

Compared to the control, trans-zeatin treatments showed significantly higher 2-AP contents of fragrant rice seedlings. Increased plant height and stem width were observed due to trans-zeatin treatments. The trans-zeatin application increased 1-pyrroline, methylglyoxal, proline, and P5C contents, enhanced P5CS and OAT activities, and reduced glutamic acid contents. In addition, expressions of ProDH, P5CS2, and DAO4 were comparatively higher under trans-zeatin treatments than CK in fragrant rice seedlings.

CONCLUSIONS

Overall, up-regulation of P5C, 1-pyrroline, and proline and down-regulation of glutamic acid under appropriate trans-zeatin concentrations (20 and 40 μmol L) resulted in enhanced 2-AP biosynthesis in fragrant rice seedlings and 20-40 μmol L was considered as the suggested concentrations of trans-zeatin application in fragrant rice seedling.

背景

在植物中，细胞分裂素被激活为玉米素，以应对非生物胁迫。然而，玉米素对香稻 2-乙酰-1-吡咯啉（2-AP）生物合成的影响机制尚未研究。本研究旨在探讨外源玉米素对香稻品种‘美香占 2 号’中 2-AP 生物合成相关酶活性、基因表达、前体物质以及 2-AP 含量和幼苗质量的影响。

结果

与对照相比，玉米素处理的香稻幼苗 2-AP 含量显著升高。玉米素处理的水稻幼苗表现出较高的株高和茎宽。玉米素处理增加了 1-吡咯啉、甲基乙二醛、脯氨酸和 P5C 的含量，增强了 P5CS 和 OAT 的活性，降低了谷氨酸的含量。此外，与 CK 相比，玉米素处理下香稻幼苗中 ProDH、P5CS2 和 DAO4 的表达水平较高。