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基于LC-QTOF-MS/MS结合化学计量学方法解析年龄对鸡胸肉汤味觉感知的影响

Decoding the Effect of Age on the Taste Perception of Chicken Breast Soup Based on LC-QTOF-MS/MS Combined with a Chemometric Approach.

作者信息

Zhang Lili, Liang Li, Qiao Kaina, Pu Dandan, Sun Baoguo, Zhou Xuewei, Zhang Yuyu

机构信息

Food Laboratory of Zhongyuan, Beijing Technology and Business University, Beijing 100048, China.

Key Laboratory of Flavor Science of China General Chamber of Commerce, Beijing Technology and Business University, Beijing 100048, China.

出版信息

Foods. 2023 Feb 3;12(3):674. doi: 10.3390/foods12030674.

Abstract

A nontargeted fingerprinting approach combined with the chemometrics method and sensory analysis was used to assess the differences in taste-chemical compositions of chicken breast soup with different ages and their sensory qualities. The sensory evaluation results showed that the overall taste as well as the sourness, saltiness, and umami scores of the soup were increased with the age of chicken. Fifty-nine compounds were identified from four soup samples by liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS), and their total content was the highest in the 90 wk soup samples. Six upregulated compounds (carnosine, hypoxanthine, inosine, inosine 5'-monophosphate (5'-IMP), adenosine 5'-monophosphate (5'-AMP), and lactic acid) were identified as potential contributors to the taste characteristics of the 90 wk soup samples by orthogonal projections to latent structures-discriminant analysis (OPLS-DA). Additional experiments showed that 5'-AMP particularly contributed to the sourness of the soup, while carnosine contributed to the saltiness and umami of the soup.

摘要

采用非靶向指纹图谱方法结合化学计量学方法和感官分析,评估不同日龄鸡胸肉汤的呈味化学成分差异及其感官品质。感官评价结果表明,汤的总体风味以及酸味、咸味和鲜味得分均随鸡肉日龄的增加而升高。通过液相色谱-串联四极杆飞行时间质谱(LC-QTOF-MS/MS)从四个汤样中鉴定出59种化合物,其总含量在90周龄汤样中最高。通过正交投影到潜在结构判别分析(OPLS-DA),确定了6种上调化合物(肌肽、次黄嘌呤、肌苷、5'-肌苷酸(5'-IMP)、5'-腺苷酸(5'-AMP)和乳酸)是90周龄汤样风味特征的潜在贡献成分。进一步实验表明,5'-AMP对汤的酸味贡献尤为显著,而肌肽则对汤的咸味和鲜味有贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf66/9914887/c5b184285e34/foods-12-00674-g001.jpg

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