Jiang Xudong, Ma Yao, Gong Sanni, Zi Xiangdong, Zhang Dawei
The Key Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu 610041, China.
College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China.
Animals (Basel). 2024 Jan 12;14(2):240. doi: 10.3390/ani14020240.
Resveratrol (RES) is a class of natural polyphenolic compounds known for its strong anti-apoptotic and antioxidant properties. Granulosa cells (GCs) are one of the important components of ovarian follicles and play crucial roles in follicular development of follicles in the ovary. Here, we explored the effects of RES on the proliferation and functions of yak GCs. Firstly, we evaluated the effect of RES dose and time in culture on the viability of GCs, and then the optimum treatment protocol (10 μM RES, 36 h) was selected to analyze the effects of RES on the proliferation, cell cycle, apoptosis, malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) accumulation, lipid droplet content, ATP production, and steroidogenesis of GCs, as well as the expression of related genes. The results show that RES treatment significantly (1) increased cell viability and proliferation and inhibited cell apoptosis by upregulating and genes and downregulating , , , and genes; (2) increased the proportion of GCs in the S phase and upregulated , , , and genes; (3) reduced ROS accumulation and MDA content and increased GSH content, as well as upregulating the relative expression levels of , , and genes; (4) decreased lipid droplet content and increased ATP production; (5) promoted progesterone (P4) secretion and the expression of P4 synthesis-related genes (, and ; and (6) inhibited E2 secretion and expression. These findings suggest that RES at 10 μM increases the proliferation and antioxidant properties, inhibits apoptosis, and promotes ATP production, lipid droplet consumption, and P4 secretion of yak GCs.
白藜芦醇（RES）是一类天然多酚化合物，以其强大的抗凋亡和抗氧化特性而闻名。颗粒细胞（GCs）是卵巢卵泡的重要组成部分之一，在卵巢卵泡的发育中起着关键作用。在此，我们探讨了RES对牦牛颗粒细胞增殖和功能的影响。首先，我们评估了培养过程中RES剂量和时间对颗粒细胞活力的影响，然后选择最佳处理方案（10 μM RES，36小时）来分析RES对颗粒细胞增殖、细胞周期、凋亡、丙二醛（MDA）、谷胱甘肽（GSH）、活性氧（ROS）积累、脂滴含量、ATP产生、类固醇生成以及相关基因表达的影响。结果表明，RES处理显著：（1）通过上调 和 基因以及下调 、 、 和 基因来提高细胞活力和增殖并抑制细胞凋亡；（2）增加颗粒细胞在S期的比例并上调 、 、 和 基因；（3）减少ROS积累和MDA含量并增加GSH含量，同时上调 、 、 和 基因的相对表达水平；（4）降低脂滴含量并增加ATP产生；（5）促进孕酮（P4）分泌以及P4合成相关基因（ 、 和 ）的表达；（6）抑制雌二醇（E2）分泌和 表达。这些发现表明，10 μM的RES可增加牦牛颗粒细胞的增殖和抗氧化特性，抑制凋亡，并促进ATP产生、脂滴消耗和P4分泌。
