He Zhiqi, Shou Chenyan, Han Zhiqiang
Fishery College, Zhejiang Ocean University, Zhoushan 316002, China.
Animals (Basel). 2023 Jan 24;13(3):400. doi: 10.3390/ani13030400.
The marbled rockfish, , belongs to the euryhaline fish and is an oviparous scleractinian fish. There are few studies on the adaptation mechanism, functional genes, and related pathways of and salinity. The results showed that a total of 72.1 GB of clean reads were obtained and all clean reads annotated a total of 25,278 Unigenes, of which 2,160 were novel genes. Compared to 20‱, 479 and 520 differential genes were obtained for 35‱ and 10‱, respectively. Gene ontology (GO) enrichment analysis revealed significant enrichment in protein binding, ion binding, ATP binding, and catalytic activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that differentially expressed genes significantly expressed under salinity stress were mainly involved in the pathways of the cytochrome P450 metabolism of xenobiotics, tryptophan metabolism, cellular senescence, and calcium signaling pathways. Among them, pik3r6b, cPLA2γ-like, and WSB1 were differentially expressed in all three groups, and they were associated with apoptosis, inflammation, DNA damage, immune regulation, and other physiological processes. Six differentially expressed genes were randomly selected for qRT-PCR validation, and the results showed that the transcriptomic data were of high confidence.
多带石斑鱼属于广盐性鱼类，是一种卵生硬骨鱼类。关于多带石斑鱼对盐度的适应机制、功能基因及相关途径的研究较少。结果显示，共获得72.1GB的 clean reads，所有clean reads共注释出25278个单基因，其中2160个为新基因。与20‰相比，在35‰和10‰条件下分别获得479个和520个差异基因。基因本体论（GO）富集分析显示，在蛋白质结合、离子结合、ATP结合和催化活性方面有显著富集。京都基因与基因组百科全书（KEGG）表明，在盐度胁迫下差异表达的基因主要参与外源性物质的细胞色素P450代谢、色氨酸代谢、细胞衰老和钙信号通路。其中，pik3r6b、cPLA2γ-like和WSB1在所有三组中均有差异表达，且它们与细胞凋亡、炎症、DNA损伤、免疫调节等生理过程相关。随机选择6个差异表达基因进行qRT-PCR验证，结果表明转录组数据具有较高可信度。
