Blokhina Inna A, Koronovskii Alexander A, Dmitrenko Alexander V, Elizarova Inna V, Moiseikina Tatyana V, Tuzhilkin Matvey A, Semyachkina-Glushkovskaya Oxana V, Pavlov Alexey N
Department of Human and Animal Physiology, Saratov State University, Astrakhanskaya Str. 83, Saratov 410012, Russia.
Physics of Open Systems Department, Saratov State University, Astrakhanskaya Str. 83, Saratov 410012, Russia.
Diagnostics (Basel). 2023 Jan 24;13(3):426. doi: 10.3390/diagnostics13030426.
Long-range correlations are often used as diagnostic markers in physiological research. Due to the limitations of conventional techniques, their characterizations are typically carried out with alternative approaches, such as the detrended fluctuation analysis (DFA). In our previous works, we found EEG-related markers of the blood-brain barrier (BBB), which limits the penetration of major drugs into the brain. However, anesthetics can penetrate the BBB, affecting its function in a dose-related manner. Here, we study two types of anesthesia widely used in experiments on animals, including zoletil/xylazine and isoflurane in optimal doses not associated with changes in the BBB. Based on DFA, we reveal informative characteristics of the electrical activity of the brain during such doses that are important for controlling the depth of anesthesia in long-term experiments using magnetic resonance imaging, multiphoton microscopy, etc., which are crucial for the interpretation of experimental results. These findings provide an important informative platform for the enhancement and refinement of surgery, since the EEG-based DFA analysis of BBB can easily be used during surgery as a tool for characterizing normal BBB functions under anesthesia.
长程相关性在生理学研究中常被用作诊断标志物。由于传统技术的局限性，其表征通常采用替代方法进行，如去趋势波动分析（DFA）。在我们之前的研究中，我们发现了与血脑屏障（BBB）相关的脑电图标志物，血脑屏障限制了主要药物进入大脑。然而，麻醉剂可以穿透血脑屏障，以剂量相关的方式影响其功能。在此，我们研究了在动物实验中广泛使用的两种麻醉剂，包括佐乐定/赛拉嗪和最佳剂量的异氟烷，这些剂量与血脑屏障的变化无关。基于DFA，我们揭示了在此类剂量下大脑电活动的信息特征，这些特征对于在使用磁共振成像、多光子显微镜等的长期实验中控制麻醉深度很重要，而这些对于解释实验结果至关重要。这些发现为手术的改进和完善提供了一个重要的信息平台，因为基于脑电图的血脑屏障DFA分析可以在手术期间轻松用作表征麻醉下正常血脑屏障功能的工具。
