Semyachkina-Glushkovskaya O V, Karavaev A S, Prokhorov M D, Runnova A E, Borovkova E I, Yu M Ishbulatov, Hramkov A N, Kulminskiy D D, Semenova N I, Sergeev K S, Slepnev A V, Yu Sitnikova E, Zhuravlev M O, Fedosov I V, Shirokov A A, Blokhina I A, Dubrovski A I, Terskov A V, Khorovodov A P, Ageev V B, Elovenko D A, Evsukova A S, Adushkina V V, Telnova V V, Postnov D E, Penzel T U, Kurths J G
Physics Department, Humboldt University, Newtonstrasse 15, 12489 Berlin, Germany.
Saratov State University, Astrakhanskaya str., 83, Saratov, 410012, Russia.
Comput Struct Biotechnol J. 2022 Dec 15;21:758-768. doi: 10.1016/j.csbj.2022.12.019. eCollection 2023.
The lymphatic drainage system of the brain (LDSB) is the removal of metabolites and wastes from its tissues. A dysfunction of LDSB is an important sign of aging, brain oncology, the Alzheimer's and Parkinson's diseases. The development of new strategies for diagnosis of LDSB injuries can improve prevention of age-related cerebral amyloid angiopathy, neurodegenerative and cerebrovascular diseases. There are two conditions, such as deep sleep and opening of the blood-brain-barrier (OBBB) associated with the LDSB activation. A promising candidate for measurement of LDSB could be electroencephalography (EEG). In this pilot study on rats, we tested the hypothesis, whether deep sleep and OBBB can be an informative platform for an effective extracting of information about the LDSB functions. Using the nonlinear analysis of EEG dynamics and machine learning technology, we discovered that the LDSB activation during OBBB and sleep is associated with similar changes in the EEG θ-activity. The OBBB causes the higher LDSB activation vs. sleep that is accompanied by specific changes in the low frequency EEG activity extracted by the power spectra analysis of the EEG dynamics combined with the coherence function. Thus, our findings demonstrate a link between neural activity associated with the LDSB activation during sleep and OBBB that is an important informative platform for extraction of the EEG-biomarkers of the LDSB activity. These results open new perspectives for the development of technology for the LDSB diagnostics that would open a novel era in the prognosis of brain diseases caused by the LDSB disorders, including OBBB.
脑淋巴引流系统（LDSB）负责从脑组织中清除代谢产物和废物。LDSB功能障碍是衰老、脑肿瘤、阿尔茨海默病和帕金森病的重要标志。开发LDSB损伤的新诊断策略可改善与年龄相关的脑淀粉样血管病、神经退行性疾病和脑血管疾病的预防。存在两种与LDSB激活相关的情况，即深度睡眠和血脑屏障开放（OBBB）。脑电图（EEG）可能是测量LDSB的一个有前景的指标。在这项针对大鼠的初步研究中，我们测试了以下假设：深度睡眠和OBBB是否可以作为一个信息平台，有效地提取有关LDSB功能的信息。通过对EEG动力学的非线性分析和机器学习技术，我们发现OBBB和睡眠期间LDSB的激活与EEG θ活动的类似变化有关。与睡眠相比，OBBB导致更高的LDSB激活，这伴随着通过EEG动力学的功率谱分析结合相干函数提取的低频EEG活动的特定变化。因此，我们的研究结果表明，睡眠和OBBB期间与LDSB激活相关的神经活动之间存在联系，这是提取LDSB活动的EEG生物标志物的重要信息平台。这些结果为LDSB诊断技术的发展开辟了新的前景，这将开启一个由LDSB紊乱（包括OBBB）引起的脑部疾病预后的新时代。
