Sugiyama T, Imai K, Yoshida H, Takayama Y, Yabana T, Yokota K, Oguma K, Yachi A
Department of Internal Medicine, Sapporo Medical College, Japan.
Gastroenterology. 1991 Jul;101(1):77-83. doi: 10.1016/0016-5085(91)90462-t.
Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to H. pylori in the sera of patients with that of normal controls by Western blot analysis, a unique antibody was detected in the sera of patients, which reacted with the 25-kilodalton antigen of H. pylori. On the other hand, monoclonal antibody CP3 prepared in the authors' laboratory also recognized the 25-kilodalton antigen of H. pylori. Whether the serum antibody of the patient recognized the CP3 antigen purified by monoclonal antibody CP3 was then examined. Western blot analysis showed that the patient's serum reacted strongly with the affinity-purified CP3 antigen. Using monoclonal antibody CP3, an enzyme-linked immunosorbent assay to detect CP3 antibody in sera was established. In patients with chronic gastritis and gastric ulcers, the titer of CP3 antibody was significantly higher than in normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful in the diagnosis of chronic gastritis and gastric ulcer associated with H. pylori infection and also in evaluation of the grade of gastritis.
幽门螺杆菌最近被认为是胃十二指肠疾病的病原体。通过蛋白质印迹分析比较幽门螺杆菌感染患者血清与正常对照血清中的幽门螺杆菌抗体，在患者血清中检测到一种独特的抗体，它与幽门螺杆菌的25千道尔顿抗原发生反应。另一方面，作者实验室制备的单克隆抗体CP3也识别幽门螺杆菌的25千道尔顿抗原。随后检测患者的血清抗体是否识别用单克隆抗体CP3纯化的CP3抗原。蛋白质印迹分析表明，患者血清与亲和纯化的CP3抗原强烈反应。利用单克隆抗体CP3，建立了一种检测血清中CP3抗体的酶联免疫吸附测定法。在慢性胃炎和胃溃疡患者中，CP3抗体滴度显著高于正常对照，且与胃窦炎的组织学分级相关。血清中CP3抗体的检测有助于诊断与幽门螺杆菌感染相关的慢性胃炎和胃溃疡，也有助于评估胃炎的分级。
