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柳叶马鞭草种子提取物的抗分枝杆菌活性。

Antimycobacterial Activity of (L.) Rusby (Malvaceae) Seed Extract.

机构信息

Department of Cell Biology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.

Laboratory of Mass Spectrometry, Intercollegiate Faculty of Biotechnology, University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.

出版信息

Cells. 2023 Jan 22;12(3):397. doi: 10.3390/cells12030397.

DOI:10.3390/cells12030397
PMID:36766739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9913413/
Abstract

The current prevalence of such lifestyle diseases as mycobacteriosis and tuberculosis is a result of the growing resistance of microorganisms to the available antibiotics and their significant toxicity. Therefore, plants can successfully become a source of new therapeutic agents. The aim of this study was to investigate the effect of protein extract from seeds on the morphology, structure, and viability of and to carry out proteomic characterization of the protein extract. The analyses were carried out using fluorescence and transmission microscopy, atomic force microscopy, and spectroscopy. The proteomic studies were performed using liquid chromatography coupled to tandem mass spectrometry. The studies showed that the seed extract applied at concentrations of 50-150 µg/mL exerted a statistically significant effect on cells, that is, a reduction of the viability of the bacteria and induction of changes in the structure of the mycobacterial cell wall. Additionally, the SEM analysis confirmed that the extract did not have a cytotoxic or cytopathic effect on fibroblast cells. The proteomic analysis revealed the presence of structural, storage, and enzymatic proteins and peptides in the extract, which are typical for seeds. Proteins and peptides with antimicrobial activity identified as vicillins and lipid-transporting proteins were also determined in the protein profile of the extract.

摘要

当前，分枝杆菌病和结核病等生活方式疾病的流行是由于微生物对现有抗生素的耐药性不断增强及其毒性显著增加所致。因此，植物可以成功成为新治疗剂的来源。本研究的目的是研究种子蛋白提取物对 和 的形态、结构和活力的影响，并对蛋白提取物进行蛋白质组学表征。使用荧光和透射显微镜、原子力显微镜和光谱法进行了分析。使用液相色谱-串联质谱法进行了蛋白质组学研究。研究表明，以 50-150 µg/mL 的浓度应用种子提取物对 细胞具有统计学显著影响，即降低了细菌的活力并诱导了分枝杆菌细胞壁结构的变化。此外，SEM 分析证实该提取物对成纤维细胞没有细胞毒性或细胞病变作用。蛋白质组学分析表明，提取物中存在结构、储存和酶蛋白和肽，这是种子的典型特征。还在提取物的蛋白质谱中确定了具有抗菌活性的蛋白和肽，如豆球蛋白和脂质转运蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/dafec845610e/cells-12-00397-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/05e47b6dc685/cells-12-00397-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/d6fee9651101/cells-12-00397-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/7f5023864ea0/cells-12-00397-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/5cc4b9931ffe/cells-12-00397-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/11840f4322d9/cells-12-00397-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/280d765dc74b/cells-12-00397-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/998d754f206d/cells-12-00397-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/05c608c08d45/cells-12-00397-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/315b76eb6300/cells-12-00397-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/dafec845610e/cells-12-00397-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/05e47b6dc685/cells-12-00397-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/d6fee9651101/cells-12-00397-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/7f5023864ea0/cells-12-00397-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/5cc4b9931ffe/cells-12-00397-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/11840f4322d9/cells-12-00397-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/280d765dc74b/cells-12-00397-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/998d754f206d/cells-12-00397-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/05c608c08d45/cells-12-00397-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/315b76eb6300/cells-12-00397-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ed8/9913413/dafec845610e/cells-12-00397-g010.jpg

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本文引用的文献

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antimycobacterial activity of medicinal plants , , and .药用植物、[此处可能有遗漏植物名称]以及[此处可能有遗漏植物名称]的抗分枝杆菌活性。
J Clin Tuberc Other Mycobact Dis. 2022 Mar 3;27:100307. doi: 10.1016/j.jctube.2022.100307. eCollection 2022 May.
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Isolation of antimicrobial peptides from different plant sources: Does a general extraction method exist?从不同植物来源分离抗菌肽：是否存在通用的提取方法？
Plant Methods. 2020 Oct 23;16:143. doi: 10.1186/s13007-020-00687-1. eCollection 2020.
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Epidemiology, diagnosis & treatment of non-tuberculous mycobacterial diseases.
非结核分枝杆菌病的流行病学、诊断与治疗。
Indian J Med Res. 2020 Sep;152(3):185-226. doi: 10.4103/ijmr.IJMR_902_20.
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A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat.脂转移蛋白具有抗真菌和抗氧化活性，并能抑制转基因小麦赤霉病和 DON 积累。
Phytopathology. 2021 Apr;111(4):671-683. doi: 10.1094/PHYTO-04-20-0153-R. Epub 2021 Mar 4.
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Features of the biochemistry of Mycobacterium smegmatis, as a possible model for Mycobacterium tuberculosis.耻垢分枝杆菌生物化学的特征，可作为结核分枝杆菌的可能模型。
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J Antibiot (Tokyo). 2020 Nov;73(11):780-789. doi: 10.1038/s41429-020-0320-7. Epub 2020 May 29.
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Sida hermaphrodita seeds as the source of anti - Candida albicans activity.麻叶荨麻籽作为抗白色念珠菌活性的来源。
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In-vitro evaluation of the antibacterial activity of the essential oils of Micromeria barbata, Eucalyptus globulus and Juniperus excelsa against strains of Mycobacterium tuberculosis (including MDR), Mycobacterium kansasii and Mycobacterium gordonae.体外评价狭叶薰衣草、蓝桉和崖柏精油对结核分枝杆菌（包括 MDR 株）、堪萨斯分枝杆菌和戈登分枝杆菌菌株的抗菌活性。
J Infect Public Health. 2019 Sep-Oct;12(5):615-618. doi: 10.1016/j.jiph.2019.01.058. Epub 2019 Feb 6.
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