Konaté Kiessoun, Zerbo Patrice, Ouédraogo Maurice, Dibala Crépin I, Adama Hilou, Sytar Oksana, Brestic Marian, Barro Nicolas
Ann Clin Microbiol Antimicrob. 2013 Jun 21;12:14. doi: 10.1186/1476-0711-12-14.
Sida urens L. (Malvaceae) is in flora of Asian medicinal herbs and used traditionally in West of Burkina Faso for the treatment of infectious diseases and particularly used against, dental caries bacteria, fever, pain and possesses analgesic properties. This study was conducted to reveal the antibacterial effect against dental caries bacteria on the one hand, and evaluate their analgesic capacity in experimental model with Swiss mice and on the other hand, with an aim to provide a scientific basis for the traditional use of this plant for the management of dental caries bacteria.
The antibacterial assays in this study were performed by using inhibition zone diameters, MIC (Minimum inhibitory concentration) and MBC (Minimal bactericidal concentration) methods. On the whole the dental caries bacteria (Gram-positive and Gram-negative bacterial strains) were used. Negative control was prepared using discs impregnated with 10% DMSO in water and commercially available Gentamicin from Alkom Laboratories LTD was used as positive reference standards for all bacterial strains. In acute toxicity test, mice received doses of extract (acetone/water extract) from Sida urens L. by intraperitoneal route and LD50 was determined in Swiss mice. As for analgesic effects, acetic acid writhing method was used in mice. The acetic acid-induced writhing method was used in mice with aim to study analgesic effects.
The results showed that the highest antibacterial activities were founded with the polyphenol-rich fractions against all bacterial strains compared to the standard antibiotic. About preliminary study in acute toxicity test, LD50 value obtained was more than 5000 mg/kg b.w. Polyphenol-rich fractions produced significant analgesic effects in acetic acid-induced writhing method and in a dose-dependent inhibition was observed.
These results validate the ethno-botanical use of Sida urens L. (Malvaceae) and demonstrate the potential of this herbaceous as a potential antibacterial agent of dental caries that could be effectively used for future health care purposes.
刺蒴麻（锦葵科）是亚洲药草植物区系的一部分，在布基纳法索西部传统上用于治疗传染病，尤其用于对抗龋齿细菌、发烧、疼痛，并具有止痛特性。本研究一方面旨在揭示其对龋齿细菌的抗菌作用，另一方面在瑞士小鼠实验模型中评估其镇痛能力，以期为该植物传统上用于管理龋齿细菌提供科学依据。
本研究中的抗菌试验采用抑菌圈直径、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）方法进行。总体上使用了龋齿细菌（革兰氏阳性和革兰氏阴性菌株）。阴性对照采用在水中浸渍10%二甲基亚砜的圆盘制备，来自Alkom Laboratories LTD的市售庆大霉素用作所有菌株的阳性参考标准。在急性毒性试验中，小鼠通过腹腔途径接受刺蒴麻提取物（丙酮/水提取物）剂量，并在瑞士小鼠中测定半数致死量（LD50）。至于镇痛效果，在小鼠中采用乙酸扭体法。采用乙酸诱导扭体法研究小鼠的镇痛效果。
结果表明，与标准抗生素相比，富含多酚的组分对所有菌株具有最高的抗菌活性。关于急性毒性试验的初步研究，获得的LD50值超过5000mg/kg体重。富含多酚的组分在乙酸诱导扭体法中产生显著的镇痛作用，并观察到剂量依赖性抑制。
这些结果证实了刺蒴麻（锦葵科）的民族植物学用途，并证明了这种草本植物作为潜在的龋齿抗菌剂的潜力，可有效用于未来的医疗保健目的。
