Kazemian Hossein, Heidari Hamid, Yamchi Jalil Kardan, Zandi Hengameh, Taji Asieh, Yazdani Fateme, Hamzehloo Gholamreza, Ghanavati Roya, Rahdar Hossein A, Feizabadi Mohammad M
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Recent Pat Antiinfect Drug Discov. 2018;13(3):240-245. doi: 10.2174/1574891X13666180626170155.
Mycobacterium tuberculosis as an intracellular pathogen causes Tuberculosis (TB). Due to the long time required for treatment, hepatotoxicity of drugs and also emergence of Multidrug-Resistant (MDR) and Extremely Drug Resistant (XDR) strains, TB is currently a major public health problem. Some medicinal plants possess remarkable activity against Mycobacterium. Among them, Lamiaceae family are of pharmaceutical interest because of their potential antimicrobial properties. The aim of the study was to evaluate the in vitro activities of Satureja rechingeri, Satureja khuzestanica and Zataria multiflora against MDR M. tuberculosis and two Non-Tuberculous Mycobacteria (NTM).
The essential oils were prepared by the standard method. The confirmed strains were obtained from the microbial collection of Tehran University of Medical Sciences. Minimum Inhibitory Concentrations (MICs) of essential oils of plants against mycobacterial strains were determined using standard broth microdilution method.
MDR M. tuberculosis was completely inhibited by Z. multiflora at 78µg/ml concentration. S. rechingeri and S. khuzestanica also showed same anti-mycobacterial activity against MDR M. tuberculosis with MICs of 156 µg/ml. The MICs of the essential oils against M. tuberculosis H37Rv, M. kansasii and M. fortuitum were in the range from 39 to 156 µg/ml.
The studied medicinal plants showed notable effects against mycobacterial strains. Our results indicated that utilization of Lamiaceae family can be helpful for treatment of mycobacterial infections.
结核分枝杆菌作为一种胞内病原体可导致结核病（TB）。由于治疗所需时间长、药物的肝毒性以及多重耐药（MDR）和广泛耐药（XDR）菌株的出现，结核病目前是一个主要的公共卫生问题。一些药用植物对分枝杆菌具有显著活性。其中，唇形科植物因其潜在的抗菌特性而具有药学研究价值。本研究的目的是评估瑞氏风轮菜、胡齐斯坦风轮菜和多花百里香对耐多药结核分枝杆菌和两种非结核分枝杆菌（NTM）的体外活性。
采用标准方法制备精油。确诊菌株来自德黑兰医科大学的微生物菌种保藏库。使用标准肉汤微量稀释法测定植物精油对分枝杆菌菌株的最低抑菌浓度（MIC）。
多花百里香在浓度为78µg/ml时可完全抑制耐多药结核分枝杆菌。瑞氏风轮菜和胡齐斯坦风轮菜对耐多药结核分枝杆菌也表现出相同的抗分枝杆菌活性，MIC为156µg/ml。精油对结核分枝杆菌H37Rv、堪萨斯分枝杆菌和偶然分枝杆菌的MIC范围为39至156µg/ml。
所研究的药用植物对分枝杆菌菌株显示出显著效果。我们的结果表明，唇形科植物的利用可能有助于治疗分枝杆菌感染。
