School of Biology and Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou 215123, China.
Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou 215123, China.
Cells. 2023 Jan 31;12(3):452. doi: 10.3390/cells12030452.
The female accumulates a large amount of egg proteins, mainly Vg and 30K, during egg formation to provide nutrition for embryo development. The synthesis and transport of Vg have been extensively studied, particularly the regulation of Vg transcription induced by 20E; however, the mechanism of 30K protein synthesis is poorly studied. As a model organism of the order Lepidoptera, has high reproduction potential. In the present study, we found that the FHL2 homologous gene () in is involved in inhibiting female egg formation by influencing the synthesis of 30K protein. Interference of expression in silkworm females increased 30K protein synthesis, accelerated ovarian development, and significantly increased the number of eggs produced and laid; however, the 20E pathway was inhibited. The transcription levels of and were significantly downregulated following overexpression in the silkworm ovarian cell line BmN. The Co-IP assay showed that the potential binding protein of BmFHL2 included three types of 30K proteins (30Kc12, 30Kc19, and 30Kc21). These results indicate that BmFHL2 participates in egg formation by affecting 30K protein in female .
雌性在卵子形成过程中积累了大量的卵蛋白，主要是 Vg 和 30K，为胚胎发育提供营养。Vg 的合成和运输已经得到了广泛的研究，特别是 20E 诱导的 Vg 转录的调节；然而，30K 蛋白合成的机制研究甚少。作为鳞翅目昆虫的模式生物，具有很高的繁殖潜力。在本研究中，我们发现家蚕中的 FHL2 同源基因（）通过影响 30K 蛋白的合成参与抑制雌性卵子形成。在家蚕雌虫中干扰表达会增加 30K 蛋白的合成，加速卵巢发育，并显著增加产卵和产蛋量；然而，20E 途径受到抑制。在蚕卵巢细胞系 BmN 中过表达后，和的转录水平显著下调。Co-IP 实验表明，BmFHL2 的潜在结合蛋白包括三种 30K 蛋白（30Kc12、30Kc19 和 30Kc21）。这些结果表明，BmFHL2 通过影响雌性中的 30K 蛋白参与卵子形成。
