Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of Korea.
College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Int J Mol Sci. 2023 Jan 22;24(3):2181. doi: 10.3390/ijms24032181.
Tomato leaf curl New Delhi virus-ES (ToLCNDV-ES), a high threat to cucurbits in the Mediterranean Basin, is listed as a different strain from the Asian ToLCNDV isolates. In this study, the infectivity of two clones previously isolated from Italy and Pakistan were compared in cucumbers, which resulted in the opposite symptom appearance. The swapping subgenome was processed; however, the mechanisms related to the disease phenotype remain unclear. To identify the disease-associated genes that could contribute to symptom development under the two ToLCNDV infections, the transcriptomes of ToLCNDV-infected and mock-inoculated cucumber plants were compared 21 days postinoculation. The number of differentially expressed genes in ToLCNDV-India-infected plants was 10 times higher than in ToLCNDV-ES-infected samples. The gene ontology (GO) and pathway enrichment were analyzed using the Cucurbits Genomics Database. The flavonoid pathway-related genes were upregulated in ToLCNDV-ES, but some were downregulated in ToLCNDV-India infection, suggesting their role in resistance to the two ToLCNDV infections. The relative expression levels of the selected candidate genes were validated by qRT-PCR under two ToLCNDV-infected conditions. Our results reveal the different infectivity of the two ToLCNDVs in cucumber and also provide primary information based on RNA-seq for further analysis related to different ToLCNDV infections.
番茄斑萎病毒-新德里 ES（ToLCNDV-ES）是地中海盆地葫芦科作物的高威胁病毒，被列为与亚洲 ToLCNDV 分离株不同的株系。本研究比较了先前从意大利和巴基斯坦分离的两个克隆在黄瓜中的感染性，导致了相反的症状表现。交换亚基因组被处理；然而，与疾病表型相关的机制仍不清楚。为了鉴定与两种 ToLCNDV 感染相关的疾病相关基因，可导致症状发展，在 ToLCNDV 感染和模拟接种的黄瓜植株中比较了 21 天后的转录组。在 ToLCNDV-印度感染的植物中差异表达基因的数量是 ToLCNDV-ES 感染样本的 10 倍。使用葫芦科基因组数据库分析基因本体论（GO）和途径富集。在 ToLCNDV-ES 中，类黄酮途径相关基因上调，但在 ToLCNDV-印度感染中一些基因下调，表明它们在抵抗两种 ToLCNDV 感染中的作用。通过 qRT-PCR 在两种 ToLCNDV 感染条件下验证了所选候选基因的相对表达水平。我们的结果揭示了两种 ToLCNDV 在黄瓜中的不同感染性，并提供了基于 RNA-seq 的初步信息，用于进一步分析不同的 ToLCNDV 感染。
