N6-甲基腺苷修饰乙型肝炎病毒 RNA 编码区。
N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of .
机构信息
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake 470-1192, Japan.
Department of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
出版信息
Int J Mol Sci. 2023 Jan 23;24(3):2265. doi: 10.3390/ijms24032265.
N6-methyladenosine (mA) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by mA predominantly in the coding region of . The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of mA by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the mA modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.
N6-甲基腺苷（mA）是 RNA 的一种转录后修饰，参与转录物的运输、降解、翻译和剪接。我们发现，HBV RNA 主要在. 的编码区被 mA 修饰。甲基化位点的突变降低了 HBV mRNA 和 HBs 蛋白水平。在原代肝细胞中用抑制剂或敲低抑制 mA 可降低培养基中的病毒 RNA 和 HBs 蛋白水平。这些结果表明，HBV RNA 的 mA 修饰对于 HBV 在肝细胞中的有效复制是必需的。
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