Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, 81377 Munich, Germany.
Department of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Wuerzburg, 97080 Wuerzburg, Germany.
Int J Mol Sci. 2023 Jan 25;24(3):2354. doi: 10.3390/ijms24032354.
Although bariatric surgery is known to change the metabolome, it is unclear if this is specific for the intervention or a consequence of the induced bodyweight loss. As the weight loss after Roux-en-Y Gastric Bypass (RYGB) can hardly be mimicked with an evenly effective diet in , translational research efforts might be helpful. A group of 188 plasma metabolites of 46 patients from the randomized controlled Würzburg Adipositas Study (WAS) and from RYGB-treated ( = 6) as well as body-weight-matched controls ( = 7) were measured using liquid chromatography tandem mass spectrometry. WAS participants were randomized into intensive lifestyle modification (LS, = 24) or RYGB (OP, = 22). In patients in the WAS cohort, only bariatric surgery achieved a sustained weight loss (BMI -34.3% (OP) vs. -1.2% (LS), ≤ 0.01). An explicit shift in the metabolomic profile was found in 57 metabolites in the cohort and in 62 metabolites in the rodent model. Significantly higher levels of sphingolipids and lecithins were detected in both surgical groups but not in the conservatively treated and animal groups. RYGB leads to a characteristic metabolomic profile, which differs distinctly from that following non-surgical intervention. Analysis of the and data revealed that RYGB induces specific changes in the metabolome independent of weight loss.
尽管减重手术已知会改变代谢组，但尚不清楚这是特定于干预措施还是体重减轻的结果。由于 Roux-en-Y 胃旁路术（RYGB）后的体重减轻几乎无法通过均匀有效的饮食来模拟，因此转化研究可能会有所帮助。使用液相色谱串联质谱法测量了来自随机对照 Würzburg 肥胖研究（WAS）的 46 名患者和 RYGB 治疗的 （= 6）以及体重匹配对照的 188 种血浆代谢物（= 7）。WAS 参与者被随机分为强化生活方式改变（LS，= 24）或 RYGB（OP，= 22）。在 WAS 队列的患者中，只有减重手术实现了持续的体重减轻（BMI -34.3%（OP）比 -1.2%（LS），≤0.01）。在 队列中发现了 57 种代谢物和在啮齿动物模型中发现了 62 种代谢物的代谢组学特征明显改变。在两个手术组中都检测到较高水平的鞘脂和卵磷脂，但在保守治疗的 和动物组中则没有。RYGB 导致特征性代谢组学特征，与非手术干预明显不同。对 和 数据的分析表明，RYGB 诱导代谢组的特定变化独立于体重减轻。
