Fujian Key Laboratory of Marine Enzyme Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China.
Int J Mol Sci. 2023 Feb 3;24(3):3004. doi: 10.3390/ijms24033004.
pv. oryzicola (Xoo) is a plant pathogenic bacterium that can cause rice bacterial blight disease, which results in a severe reduction in rice production. Antimicrobial-dependent microbial controlling is a useful way to control the spread and outbreak of plant pathogenic bacteria. However, the abuse and long-term use of antimicrobials also cause microbial antimicrobial resistance. As far as known, the mechanism of antimicrobial resistance in agricultural plant pathogenic bacteria still lacks prospecting. In this study, we explore the mechanism of Zhongshengmycin (ZSM)-resistance in Xoo by GC-MS-based metabolomic analysis. The results showed that the down-regulation of the TCA cycle was characteristic of antimicrobial resistance in Xoo, which was further demonstrated by the reduction of activity and gene expression levels of key enzymes in the TCA cycle. Furthermore, alanine was proven to reverse the ZSM resistance in Xoo by accelerating the TCA cycle in vivo. Our results are essential for understanding the mechanisms of ZSM resistance in Xoo and may provide new strategies for controlling this agricultural plant pathogen at the metabolic level.
水稻欧文氏菌（Xoo）是一种植物病原细菌，可引起水稻细菌性条斑病，导致水稻严重减产。抗菌依赖型微生物控制是控制植物病原细菌传播和爆发的有效方法。然而，抗菌药物的滥用和长期使用也会导致微生物对抗菌药物的耐药性。据目前所知，农业植物病原细菌的抗菌药物耐药性机制仍缺乏探索。在这项研究中，我们通过 GC-MS 代谢组学分析探索了中生菌素（ZSM）在 Xoo 中耐药性的机制。结果表明，TCA 循环的下调是 Xoo 抗菌药物耐药性的特征，这进一步通过 TCA 循环关键酶的活性和基因表达水平的降低得到证实。此外，通过在体内加速 TCA 循环，证明了丙氨酸可逆转 Xoo 对 ZSM 的耐药性。我们的研究结果对于理解 Xoo 中 ZSM 耐药性的机制至关重要，并可能为在代谢水平上控制这种农业植物病原菌提供新的策略。
