Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement "Moskovskiy", 108819 Moscow, Russia.
Int J Mol Sci. 2023 Feb 3;24(3):3020. doi: 10.3390/ijms24033020.
Forty-five strains of AIVs were isolated from wild aquatic birds during their autumn migration through Moscow (Russia). The aim of this work is to study the dynamics of AIV genomes in their natural habitat. Viruses were isolated from fecal sample in embryonated chicken eggs; their complete genomes were sequenced, and a phylogenetic analysis was performed. The gene segments of the same lineage persisted over the years in the absence of persistence of complete viral genomes. The genes for internal proteins of the same lineage were often maintained by the viruses over few years; however, they were typically associated with the genes of novel HA and NA subtypes. Although frequent reassortment events were observed for any pair of internal genes, there was no reassortment between HA and NA segments. The differences in the persistence of phylogenetic lineages of surface and internal proteins and the different evolutionary strategy for these two types of genes of AIVs in primary hosts are discussed.
从秋季迁徙经过莫斯科（俄罗斯）的野生水鸟中分离出了 45 株 AIVs。本工作旨在研究其天然栖息地中 AIV 基因组的动态变化。从禽胚蛋粪便样本中分离出病毒；对其完整基因组进行测序，并进行系统发育分析。在没有完整病毒基因组持续存在的情况下，相同谱系的基因片段多年来一直持续存在。相同谱系的内部蛋白基因通常在几年内被病毒维持，但它们通常与新型 HA 和 NA 亚型的基因相关。尽管经常观察到任何一对内部基因的重组事件，但 HA 和 NA 片段之间没有重组。讨论了表面蛋白和内部蛋白的系统发育谱系的持久性差异以及宿主中这两种类型的 AIVs 基因的不同进化策略。
