Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.
Ophthalmology Clinic, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.
Int J Mol Sci. 2023 Feb 3;24(3):3032. doi: 10.3390/ijms24033032.
Proliferative vitreoretinopathy (PVR) is an abnormal intraocular scarring process that can complicate cases of rhegmatogenous retinal detachment (RRD). Although previous studies have examined the relevance of microRNAs (miRNAs) in ophthalmic diseases, only a few studies have evaluated the expression profiles of microRNAs in subretinal fluid. We hypothesized that the expression profiles of specific miRNAs may change in response to RRD, in the subretinal fluid that is directly in contact with photoreceptors and the retinal pigment epithelium (RPE). We looked for a potential correlation between the expression of specific miRNAs in eyes with RRD and known clinical risk factors of PVR. A total of 24 patients (59 ± 11 years) who underwent scleral buckling procedure were enrolled in this prospective study. Twenty-four undiluted subretinal fluid samples were collected, RNA was isolated and qRT-PCR was performed to analyze the expression of 12 miRNAs. We found the existence of a positive association between the expression of miR-21 ( = 0.017, r = 0.515) and miR-34 ( = 0.030, r = 0.624) and the duration of symptoms related to retinal detachment. Moreover, the expression of miR-146a tended to decrease in patients who developed PVR. Subretinal fluid constitutes an intriguing biological matrix to evaluate the role of miRNAs leading to the development of PVR.
增生性玻璃体视网膜病变（PVR）是一种异常的眼内瘢痕形成过程，可使孔源性视网膜脱离（RRD）的病例复杂化。虽然之前的研究已经检查了 microRNAs（miRNAs）在眼科疾病中的相关性，但只有少数研究评估了视网膜下液中 microRNAs 的表达谱。我们假设，在与光感受器和视网膜色素上皮（RPE）直接接触的视网膜下液中，特定 miRNAs 的表达谱可能会因 RRD 而发生变化。我们在患有 RRD 的眼睛中寻找特定 miRNAs 的表达与已知的 PVR 临床危险因素之间的潜在相关性。这项前瞻性研究共纳入了 24 名（59 ± 11 岁）接受巩膜扣带术的患者。共收集了 24 份未稀释的视网膜下液样本，提取 RNA 并进行 qRT-PCR 分析以分析 12 种 miRNAs 的表达。我们发现 miR-21（ = 0.017，r = 0.515）和 miR-34（ = 0.030，r = 0.624）的表达与视网膜脱离相关症状的持续时间之间存在正相关关系。此外，miR-146a 的表达在发生 PVR 的患者中呈下降趋势。视网膜下液构成了一个有趣的生物基质，可以评估导致 PVR 发生的 miRNAs 的作用。
